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[Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog]

[Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog] Research Abstract Details 

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  • [Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog] Abstract Text:

    hao wenHao Wen,ya-lou zhangYa-lou Zhang,jean-mathieu bartJean-Mathieu Bart,p giraudouxP Giraudoux,d a vuittonD A Vuitton,xu-dong maXu-dong Ma,lin-yue zouLin-yue Zou,yu-qing miaoYu-qing Miao,p s craigP S Craig,

    OBJECTIVE: To identify mixed infection of Echinococcus granulosus and E. multilocularis in a dog from Xinjiang. METHODS: Thirty dogs from the pasture area were dissected and over 10,000 Echinococcus adult worms were found from one dog. Morphological observation revealed possible mixed infection of the two Echinococcus species. Further identifcation was made by amplification of the target gene DNA fragment (mitochondrial 12S rRNA gene). RESULTS: The adult worms of E. granulosus showed a relatively longer and larger gravid proglottid, its genital pore situated near or below the middle-side of the segment. The uterus was in a sacculate shape with irregular branches and approximately over 200 - 800 eggs in it. Morphology of the adult worms of E. multilocularis was similar to E. granulosus, slightly smaller, consisting of 4 to 5 proglottids. The uterus was not sacculate and with no branch. Its lateral genital pore often situated in the anterior part of the segment. Sequence analysis of mitochondrial 12S rRNA gene showed that amplification with the Eg1f/r primers shared complete identity with E. granulosus G1 genotype (GentBank accession no. AY462129), while that witht the EmH15/17 primers shared complete identity with E. multilocularis (GenBank accession no. AB031351). The presence of both E. granulosus and E. multicularis was confirmed by microscopy and gene identification. CONCLUSION: Mixed infection of the two species of Echinococcus has been confirmed in the dog by morphological observation and PCR technique.

    [Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog] Publishing Authors By Initials

    h wenH Wen,yl zhangYL Zhang,jm bartJM Bart,p giraudouxP Giraudoux,da vuittonDA Vuitton,xd maXD Ma,ly zouLY Zou,yq miaoYQ Miao,ps craigPS Craig,

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    [Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog] Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Zhongguo ji sheng chong xue yu ji sheng chong bing

    VOLUME: 24

    Page Numbers: 10-3

    Journal Abbreviation:

    ISSN: 1000-7423

    DAY: 28

    MONTH: Feb

    YEAR: 2006

    [Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog] Information

    Number of References:

    LANGUAGE: chi

    NlmUniqueID: 8709992

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    Grant and Affiliation Information for [Mixed infection of Echinococcus granulosus and Echinococcus multilocularis in dog]

    AFFILIATION: Xinjiang Hydatid Clinical Research Institute, Urumqi 830054, China.

    Country: China

    China Research PublicationChina Research Publication

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    MEDLINETA: Zhongguo Ji Sheng Chong Xue Yu

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