Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression.

Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression. Research Abstract Details 

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Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression. Abstract Text:

Nancy Nutile-McMenemy,Arye Elfenbein,Joyce A Deleo,Nancy Nutile-McMenemy,Arye Elfenbein,Joyce A Deleo,

Minocycline is a semisynthetic, tetracycline derivative that exerts anti-inflammatory and neuroprotective effects unrelated to its anti-microbial action. We have previously shown that minocycline prevented peripheral nerve injury-induced mechanical allodynia. Minocycline's mechanisms of action as a neuroprotective and anti-allodynic agent are unknown. In response to injury, microglia become activated, proliferate, and migrate. Resting microglia express voltage-dependent inward K(+) currents and blocking Kv1.3 channels has been shown to inhibit microglial-mediated neuronal death. We investigated the effect of minocycline on the expression of Kv channels, cell motility, and beta-integrin expression using primary rat cortical microglia, transwell assays, and by flow cytometry. Minocycline significantly reduced microglial migration to cellular debris, astrocyte-conditioned medium, ADP, and algesic mediators and significantly reduced the expression of CD29 (beta(1)-integrin) but not CD18 (beta(2)-integrin). Minocycline reduced the effect of extracellular potassium and later decreased microglial Kv1.3 expression. In summary, we uncovered a novel effect of minocycline that demonstrates this agent decreases microglial beta(1)-integrin expression, which leads to inhibition of motility. We propose an in vivo model whereby reduced microglial trafficking to injured neurons following nerve injury decreases the release of proinflammatory mediators into the synaptic milieu, preventing neuronal sensitization, the pathological correlate to chronic pain.

Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression. Publishing Authors By Initials

N Nutile-McMenemy,A Elfenbein,JA Deleo,N Nutile-McMenemy,A Elfenbein,JA Deleo,

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Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neurochemistry

VOLUME: 103

Page Numbers: 2035-46

Journal Abbreviation: J. Neurochem.

ISSN: 1471-4159

DAY: 14

MONTH: 09

YEAR: 2007

Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression. Information

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LANGUAGE: eng

NlmUniqueID: 2985190

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Grant and Affiliation Information for Minocycline decreases in vitro microglial motility, beta1-integrin, and Kv1.3 channel expression.

AFFILIATION: Department of Anesthesiology, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA.

Country: England

AGENCY: United States NIDA

GRANT: DA11276

ACRONYM: DA

MEDLINETA: J Neurochem

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