Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct.

Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Research Abstract Details 

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Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Abstract Text:

Vincent Falanga,Janet Butmarc,Jisun Cha,Tatyana Yufit,Polly Carson,

A bilayered bioengineered living skin construct (LSC) consisting of viable human neonatal keratinocytes over a collagenous dermis seeded with dermal fibroblasts has been used extensively in difficult-to heal human wounds. Its biological properties include production of several mediators, cytokines, and growth factors and the ability to heal itself upon injury. In this study, we investigated the process of keratinocyte migration in LSC. At baseline, 6-mm punch biopsies of the construct were placed in serum-free medium (AIM-V) or Dulbecco's modified Eagle medium. At varying time points, the LSC samples were processed and analyzed using histology and immunohistochemistry. By 72 h, in a time-dependent manner, the overlying epidermis had migrated over and enveloped the entire underlying dermis, a process known as epiboly. Increasing concentrations of neutralizing antibodies to epidermal growth factor or interleukin-1 alpha down-regulated the extent of epiboly, as measured using computerized planimetry, but antibodies to transforming growth factor-beta 1 did not affect it. The consistent expression of laminin V, alpha3beta1 integrin, and vitronectin (epibolin) and its integrin receptor (alphavbeta5) characterized the tongue of migrating epidermis. Increasing concentrations of antibodies to vitronectin blocked the process of epiboly, as did antibodies to the alphavbeta5 integrin receptor, which mediates vitronectin-driven keratinocyte locomotion. This process of epiboly provides novel mechanisms of action for bioengineered skin constructs.

Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Publishing Authors By Initials

V Falanga,J Butmarc,J Cha,T Yufit,P Carson,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research

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Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Tissue engineering

VOLUME: 13

Page Numbers: 21-8

Journal Abbreviation: Tissue Eng.

ISSN: 1076-3279

DAY: 3

MONTH: Jan

YEAR: 2007

Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9505538

Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Keywords Mesh Terms:

KEYWORDS: Tissue Engineering

MESH TERMS: physiology

Chemical & Substance for Abstract: Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct. Information

Substance Name: Collagen

Registry Number: 9007-34-5

Grant and Affiliation Information for Migration of the epidermal over the dermal component (epiboly) in a bilayered bioengineered skin construct.

AFFILIATION: Department of Dermatology, Roger Williams Medical Center, Providence, Rhode Island 02908, and Boston University School of Medicine, Boston, Massachusetts, USA. vfalanga@bu.edu

Country: United States

AGENCY: United States NIDDK

GRANT: DK067836

ACRONYM: DK

MEDLINETA: Tissue Eng

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