Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties.

Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Research Abstract Details 

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Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Abstract Text:

M Tamura,T Yubisui,M Takeshita,

Bovine brain microsomal NADH-cytochrome b5 (cyt. b5) reductase [EC 1.6.2.2] was solubilized by digestion with lysosomes, and purified 8,500-fold with a 20% recovery by procedures including affinity chromatography on 5'-AMP-Sepharose 4B. The purified enzyme showed one band of a molecular weight of 31,000 on polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS). Polyacrylamide gel electrophoresis of the purified enzyme without SDS revealed a major band with a faint minor band, both of which exhibited NADH-cyt. b5 reductase activity. The isoelectric points of these components were 6.0 (major) and 6.3 (minor). The apparent Km values of the purified enzyme for NADH and ferricyanide were 1.1 and 4.2 microM, respectively. The apparent Km value for cyt. b5 was 14.3 microM in 10 mM potassium phosphate buffer (pH 7.5). The apparent Vmax value was 1,190 mumol cyt. b5 reduced/min/mg of protein. The NADH-cyt. b5 reductase activity of the purified enzyme was inhibited by sulfhydryl inhibitors and flavin analogues. Inhibition by phosphate buffer or other inorganic salts of the enzyme activity of the purified enzyme was proved to be of the competitive type. These properties were similar to those of NADH-cyt. b5 reductase from bovine liver microsomes or rabbit erythrocytes, although the estimated enzyme content in brain was about one-twentieth of that in liver (per g wet tissue). An immunochemical study using an antibody to purified NADH-cyt. b5 reductase bovine liver microsomes indicated that NADH-cyt. b5 reductase from brain microsomes is immunologically identical to the liver microsomal enzyme.

Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Publishing Authors By Initials

M Tamura,T Yubisui,M Takeshita,

For similar proteins: nerve tissue proteins research abstracts see: proteins: nerve tissue proteins research

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Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 1547-55

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1983

Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Keywords Mesh Terms:

KEYWORDS: Nerve Tissue Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Microsomal NADH-cytochrome b5 reductase of bovine brain: purification and properties. Information

Substance Name: Cytochrome-B(5) Reductase

Registry Number: EC 1.6.2.2

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Country: JAPAN

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MEDLINETA: J Biochem

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