Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides.

Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Research Abstract Details 

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Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Abstract Text:

Toshikazu Minamisawa,Kiyoshi Suzuki,Naoko Kajimoto,Masami Iida,Hiroshi Maeda,Jun Hirabayashi,

In order to prepare a series of N-acetylheparosan (NAH)-related oligosaccharides, bacterial NAH produced in Escherichia coli strain K5 was partially depolymerized with heparitinase I into a mixture of even-numbered NAH oligosaccharides, having an unsaturated uronic acid (DeltaUA) at the non-reducing end. A mixture of odd-numbered oligosaccharides was derived by removing this DeltaUA in the aforementioned mixture by a 'trimming' reaction using mercury(II) acetate. Each oligosaccharide mixture was subjected to gel-filtration chromatography to generate a series of size-uniform NAH oligosaccharides of satisfactory purity (assessed by analytical anion-exchange HPLC), and their structures were identified by MALDITOF-MS, ESIMS, and 1H NMR analysis. As a result, a microscale preparation of a series of both even- and odd-numbered NAH oligosaccharides was achieved for the first time. The developed procedure is simple and systematic, and thus, should be valuable for providing not only research tools for heparin/heparan sulfate-specific enzymes and their binding proteins, but also precursor substrates with medical applications.

Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Publishing Authors By Initials

T Minamisawa,K Suzuki,N Kajimoto,M Iida,H Maeda,J Hirabayashi,

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Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Carbohydrate research

VOLUME: 341

Page Numbers: 230-7

Journal Abbreviation: Carbohydr. Res.

ISSN: 0008-6215

DAY: 5

MONTH: 12

YEAR: 2005

Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Information

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LANGUAGE: eng

NlmUniqueID: 43535

Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Keywords Mesh Terms:

KEYWORDS: Oligosaccharides

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides. Information

Substance Name: Acetylglucosamine

Registry Number: 7512-17-6

Grant and Affiliation Information for Microscale preparation of even- and odd-numbered N-acetylheparosan oligosaccharides.

AFFILIATION: Glycostructure Analysis Team, Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology (AIST), Central-2, 1-1-1 Umezono, Tsukuba, Ibaraki 305-8568, Japan.

Country: Netherlands

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MEDLINETA: Carbohydr Res

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