MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision.

MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision. Research Abstract Details 

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MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision. Abstract Text:

The elucidation of the architecture of gene regulatory networks that control cell-type specific gene expression programs represents a major challenge in developmental biology. We describe here a cell fate decision between two alternative neuronal fates and the architecture of a gene regulatory network that controls this cell fate decision. The two Caenorhabditis elegans taste receptor neurons "ASE left" (ASEL) and "ASE right" (ASER) share many bilaterally symmetric features, but each cell expresses a distinct set of chemoreceptors that endow the gustatory system with the capacity to sense and discriminate specific environmental inputs. We show that these left/right asymmetric fates develop from a precursor state in which both ASE neurons express equivalent features. This hybrid precursor state is unstable and transitions into the stable ASEL or ASER terminal end state. Although this transition is spatially stereotyped in wild-type animals, mutant analysis reveals that each cell has the potential to transition into either the ASEL or ASER stable end state. The stability and irreversibility of the terminal differentiated state is ensured by the interactions of two microRNAs (miRNAs) and their transcription factor targets in a double-negative feedback loop. Simple feedback loops are found as common motifs in many gene regulatory networks, but the loop described here is unusually complex and involves miRNAs. The interaction of miRNAs in double-negative feedback loops may not only be a means for miRNAs to regulate their own expression but may also represent a general paradigm for how terminal cell fates are selected and stabilized.

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MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 102

Page Numbers: 12449-54

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 12

MONTH: 08

YEAR: 2005

MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision. Keywords Mesh Terms:

KEYWORDS: Receptors, G-Protein-Coupled

MESH TERMS: metabolism

Chemical & Substance for Abstract: MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision. Information

Substance Name: taste receptors, type 1

Registry Number: 0

Grant and Affiliation Information for MicroRNAs acting in a double-negative feedback loop to control a neuronal cell fate decision.

AFFILIATION: Department of Biochemistry and Molecular Biophysics, Center for Neurobiology and Behavior, Howard Hughes Medical Institute, Columbia University Medical Center, New York, NY 10032, USA.

Country: United States

AGENCY: United States NINDS

GRANT: R01 NS050266-05

ACRONYM: NS

MEDLINETA: Proc Natl Acad Sci U S A

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