Microgram-scale protein structure determination by NMR.

Microgram-scale protein structure determination by NMR. Research Abstract Details 

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Microgram-scale protein structure determination by NMR. Abstract Text:

James M Aramini,Paolo Rossi,Clemens Anklin,Rong Xiao,Gaetano T Montelione,

Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mug (6 microl, 1.4 mM) of protein. This first example of a complete solution NMR structure determined using microgram quantities of protein demonstrates the utility of microcoil-probe NMR technologies for protein samples that can be produced in only limited quantities.

Microgram-scale protein structure determination by NMR. Publishing Authors By Initials

JM Aramini,P Rossi,C Anklin,R Xiao,GT Montelione,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: molecular conformation: protein conformation research

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Microgram-scale protein structure determination by NMR. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nature methods

VOLUME: 4

Page Numbers: 491-3

Journal Abbreviation: Nat. Methods

ISSN: 1548-7091

DAY: 13

MONTH: 05

YEAR: 2007

Microgram-scale protein structure determination by NMR. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101215604

Microgram-scale protein structure determination by NMR. Keywords Mesh Terms:

KEYWORDS: Protein Conformation

MESH TERMS: chemistry

Chemical & Substance for Abstract: Microgram-scale protein structure determination by NMR. Information

Substance Name: Archaeal Proteins

Registry Number: 0

Grant and Affiliation Information for Microgram-scale protein structure determination by NMR.

AFFILIATION: Center for Advanced Biotechnology and Medicine, Department of Molecular Biology and Biochemistry, and Northeast Structural Genomics Consortium (NESG), Rutgers University, Piscataway, New Jersey 08854, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: U54 GM074958

ACRONYM: GM

MEDLINETA: Nat Methods

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