Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay.

Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay. Research Abstract Details 

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Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay. Abstract Text:

Kuo-Hoong Lee,Yuan-Deng Su,Shean-Jen Chen,Fan-Gang Tseng,Gwo-Bin Lee,Kuo-Hoong Lee,Yuan-Deng Su,Shean-Jen Chen,Fan-Gang Tseng,Gwo-Bin Lee,

This study reports a microfluidic chip integrated with an arrayed immunoassay for surface plasmon resonance (SPR) phase imaging of specific bio-samples. The SPR phase imaging system uses a surface-sensitive optical technique to detect two-dimensional (2D) spatial phase variation caused by rabbit immunoglobulin G (IgG) adsorbed on an anti-rabbit IgG film. The microfluidic chip was fabricated by using micro-electro-mechanical-systems (MEMS) technology on glass and polydimethylsiloxane (PDMS) substrates to facilitate well-controlled and reproducible sample delivery and detection. Since SPR detection is very sensitive to temperature variation, a micromachine-based temperature control module comprising micro-heaters and temperature sensors was used to maintain a uniform temperature distribution inside the arrayed detection area with a variation of less than 0.3 degrees C. A self-assembled monolayer (SAM) technique was used to pattern the surface chemistry on a gold layer to immobilize anti-rabbit IgG on the modified substrates. The microfluidic chip is capable of transporting a precise amount of IgG solution by using micropumps/valves to the arrayed detection area such that highly sensitive, highly specific bio-sensing can be achieved. The developed microfluidic chips, which employed SPR phase imaging for immunoassay analysis, could successfully detect the interaction of anti-rabbit IgG and IgG. The interactions between immobilized anti-rabbit IgG and IgG with various concentrations have been measured. The detection limit is experimentally found to be 1x10(-4)mg/ml (0.67nM). The specificity of the arrayed immunoassay was also explored. Experimental data show that only the rabbit IgG can be detected and the porcine IgG cannot be adsorbed. The developed microfluidic system is promising for various applications including medical diagnostics, microarray detection and observing protein-protein interactions.

Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay. Publishing Authors By Initials

KH Lee,YD Su,SJ Chen,FG Tseng,GB Lee,KH Lee,YD Su,SJ Chen,FG Tseng,GB Lee,

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Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biosensors & bioelectronics

VOLUME: 23

Page Numbers: 466-72

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ISSN: 0956-5663

DAY: 2

MONTH: 06

YEAR: 2007

Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Microfluidic systems integrated with two-dimensional surface plasmon resonance phase imaging systems for microarray immunoassay.

AFFILIATION: Department of Engineering Science, National Cheng Kung University, Taiwan.

Country: England

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MEDLINETA: Biosens Bioelectron

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