Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins.

Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins. Research Abstract Details 

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Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins. Abstract Text:

Abdulilah A Dawoud,Hetal A Sarvaiya,Iulia M Lazar,Abdulilah A Dawoud,Hetal A Sarvaiya,Iulia M Lazar,Abdulilah A Dawoud,Hetal A Sarvaiya,Iulia M Lazar,

The development of novel and reliable technologies for the analysis of proteins and their post-translational modifications, in particular, has recently received much attention and interest. The implementation of a fully integrated microfluidic device interfaced with MS detection for the analysis of phosphoproteins is presented in this paper. The microfluidic platform (3''x1.5'') comprises two individual sample processing systems: one for performing direct sample infusion and one for performing microfluidic LC separations. Various MS detection strategies, specific for the study of post-translational modifications, were conducted using alpha-casein as a model protein. Neutral loss ion mapping, data-dependent triple-play and neutral loss analysis, and in situ dephosphorylation followed by LC separation and MS detection were performed. Consistent results in identifying phosphopeptides with conventional and microfluidic instrumentation have been obtained. Unlike with conventional instrumentation, however, the microfluidic device enabled the completion of each analysis from only a few microliters of sample, in approximately 10-15 min, and on a bioanalytical platform that facilitates multiplexing and disposability, and thus high-throughput, contamination-free analysis.

Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins. Publishing Authors By Initials

AA Dawoud,HA Sarvaiya,IM Lazar,AA Dawoud,HA Sarvaiya,IM Lazar,AA Dawoud,HA Sarvaiya,IM Lazar,

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Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Electrophoresis

VOLUME: 28

Page Numbers: 4645-60

Journal Abbreviation: Electrophoresis

ISSN: 0173-0835

DAY: 17

MONTH: Dec

YEAR: 2007

Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Microfluidic platform with mass spectrometry detection for the analysis of phosphoproteins.

AFFILIATION: Virginia Bioinformatics Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

Country: Germany

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MEDLINETA: Electrophoresis

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