Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells.

Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells. Research Abstract Details 

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Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells. Abstract Text:

Nanae Fujimoto,Satoshi Fujita,Takashi Tsuji,Junya Toguchida,Kenji Ida,Hiroshi Suginami,Hiroo Iwata,Nanae Fujimoto,Satoshi Fujita,Takashi Tsuji,Junya Toguchida,Kenji Ida,Hiroshi Suginami,Hiroo Iwata,

Expansion of hematopoietic stem cells (HSCs) from cord blood is highly desired for treatment and transplantation of adult patients for hematologic diseases. For efficient proliferation of HSCs, CD34(+) cells from cord blood were co-cultured with microencapsulated murine stromal cells (HESS-5) or immortalized human mesenchymal stem cells (MSCs) in their conditioned media (CM). Bioactive substances for HSC proliferation in CM at the onset of culture are likely consumed by HSCs with time, and co-culturing with microencapsulated feeder cells ensures a continuous supply. The cell number of CD34(+) cell progeny efficiently increased under these culture conditions, and progeny were analyzed by flow cytometry, the colony assay and the cobblestone area-forming cell (CAFC) assay. Total nucleated cells and CD34(+) cell number increased 194- and 7.4-fold, respectively, in the presence of microencapsulated HESS-5 in CM. Colony forming cells and CAFCs were well maintained. The effective expansion of total cells and maintenance of primitive progenitor cells suggest that transfusion of the progeny obtained from CD34(+) cell culture with microencapsulated HESS-5 in CM could shorten the time to engraftment by bridging the pancytopenic period and support functional hematopoietic repopulation.

Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells. Publishing Authors By Initials

N Fujimoto,S Fujita,T Tsuji,J Toguchida,K Ida,H Suginami,H Iwata,N Fujimoto,S Fujita,T Tsuji,J Toguchida,K Ida,H Suginami,H Iwata,

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Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomaterials

VOLUME: 28

Page Numbers: 4795-805

Journal Abbreviation: Biomaterials

ISSN: 0142-9612

DAY: 9

MONTH: 08

YEAR: 2007

Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 8100316

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Grant and Affiliation Information for Microencapsulated feeder cells as a source of soluble factors for expansion of CD34(+) hematopoietic stem cells.

AFFILIATION: Department of Regenerative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.

Country: England

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MEDLINETA: Biomaterials

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