Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis.

Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis. Research Abstract Details 

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Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis. Abstract Text:

Bisi Lawuyi,Hongmei Chen,Fatemeh Afkhami,Arun Kulamarva,Satya Prakash,Bisi Lawuyi,Hongmei Chen,Fatemeh Afkhami,Arun Kulamarva,Satya Prakash,

This article demonstrates the potential of encapsulated, engineered Lactococcus lactis as a vehicle for the oral delivery of therapeutic proteins. Using alginate-poly-l-lysine-alginate membrane-encapsulated L. lactis engineered to secrete the reporter protein Staphylococcal aureus nuclease, we show comparable viability and protein secretion between free and immobilized cells. After 12 h, microcapsules with a cell density of 4.8 x 10(5) colony forming unit (CFU) ml(-1) grew to 2.2 x 10(8) CFU ml(-1) and released 0.24 arbitrary unit (AU) ml(-1) of nuclease, producing similar results as free cells, which grew from 3.4 x 10(5) to 1.9 x 10(8) CFU ml(-1) and secreted 0.21 AU ml(-1) of nuclease. Moreover, encapsulated cells at a density of 4.4 x 10(7) CFU ml(-1) grew to 2.2 x 10(10) CFU ml(-1) in 12 h and secreted 15.3 AU ml(-1) of nuclease although 3.1 x 10(7) CFU ml(-1)of free cells reached only 2.3 x 10(9) CFU ml(-1) and released 5.6 AU ml(-1) of nuclease. We also show the sustained stability of the microcapsules during storage at 4 degrees C over 8 weeks.

Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis. Publishing Authors By Initials

B Lawuyi,H Chen,F Afkhami,A Kulamarva,S Prakash,B Lawuyi,H Chen,F Afkhami,A Kulamarva,S Prakash,

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Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Applied biochemistry and biotechnology

VOLUME: 142

Page Numbers: 71-80

Journal Abbreviation: Appl. Biochem. Biotechnol.

ISSN: 0273-2289

DAY: 20

MONTH: Jul

YEAR: 2007

Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis. Information

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LANGUAGE: eng

NlmUniqueID: 8208561

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Grant and Affiliation Information for Microencapsulated engineered Lactococcus lactis cells for heterologous protein delivery: preparation and in vitro analysis.

AFFILIATION: Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering and Artificial Cells and Organs Research Center, Faculty of Medicine, McGill University, 3775 University Street, Montreal, Quebec, Canada.

Country: United States

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MEDLINETA: Appl Biochem Biotechnol

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