Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo.

Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Research Abstract Details 

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Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Abstract Text:

Holly M Shackman,Minshan Shou,Nicholas A Cellar,Christopher J Watson,Robert T Kennedy,

Capillary liquid chromatography-mass spectrometry (cLC-MS) was coupled on-line to microdialysis sampling to monitor endogenous acetylcholine (ACh) from the rodent brain. In vivo microdialysate sampled at 0.6 microL/min from the striatum of ketamine or chloral hydrate anesthetized rats was loaded onto a sample loop and then injected onto a approximately 5 cm long strong cation exchange (SCX) capillary column. A step gradient was used to separate the analyte from ionization suppressing salts contained in dialysate in 2.4 min. Sampling coupled on-line with cLC-MS allowed for high temporal resolution (data points at 2.4 min intervals), good reproducibility (10-15% relative standard deviation, R.S.D.), and sensitive limits of detection (0.04 nM or 8 amol injected). The method successfully monitored basal and stimulated levels (induced by increased K+ concentrations) of ACh from the anesthetized rat without necessitating perfusion of an acetylcholinesterase (AChE) inhibitor. Absolute and percent basal levels of ACh from rats receiving different anesthetics were also compared.

Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Publishing Authors By Initials

HM Shackman,M Shou,NA Cellar,CJ Watson,RT Kennedy,

For similar investigative techniques: epidemiologic methods: epidemiologic research design: reproducibility of results research abstracts see: investigative techniques: epidemiologic methods: epidemiologic research design: reproducibility of results research

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Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of neuroscience methods

VOLUME: 159

Page Numbers: 86-92

Journal Abbreviation: J. Neurosci. Methods

ISSN: 0165-0270

DAY: 28

MONTH: 07

YEAR: 2006

Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905558

Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Keywords Mesh Terms:

KEYWORDS: Reproducibility of Results

MESH TERMS: methods

Chemical & Substance for Abstract: Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo. Information

Substance Name: Ketamine

Registry Number: 6740-88-1

Grant and Affiliation Information for Microdialysis coupled on-line to capillary liquid chromatography with tandem mass spectrometry for monitoring acetylcholine in vivo.

AFFILIATION: Department of Chemistry, University of Michigan, Ann Arbor, MI 48109-1055, USA.

Country: Netherlands

AGENCY: United States NINDS

GRANT: NS38476

ACRONYM: NS

MEDLINETA: J Neurosci Methods

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