Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury.

Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury. Research Abstract Details 

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Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury. Abstract Text:

Brett A Simon,R Blaine Easley,Dmitry N Grigoryev,Shwu-Fan Ma,Shui Q Ye,Tera Lavoie,Rubin M Tuder,Joe G N Garcia,

Human acute lung injury is characterized by heterogeneous tissue involvement, leading to the potential for extremes of mechanical stress and tissue injury when mechanical ventilation, required to support critically ill patients, is employed. Our goal was to establish whether regional cellular responses to these disparate local mechanical conditions could be determined as a novel approach toward understanding the mechanism of development of ventilator-associated lung injury. We utilized cross-species genomic microarrays in a unilateral model of ventilator-associated lung injury in anesthetized dogs to assess regional cellular responses to local mechanical conditions that potentially contribute pathogenic mechanisms of injury. Highly significant regional differences in gene expression were observed between lung apex/base regions as well as between gravitationally dependent/nondependent regions of the base, with 367 and 1,544 genes differentially regulated between these regions, respectively. Major functional groupings of differentially regulated genes included inflammation and immune responses, cell proliferation, adhesion, signaling, and apoptosis. Expression of genes encoding both acute lung injury-associated inflammatory cytokines and protective acute response genes were markedly different in the nondependent compared with the dependent regions of the lung base. We conclude that there are significant differences in the local responses to stress within the lung, and consequently, insights into the cellular responses that contribute to ventilator-associated lung injury development must be sought in the context of the mechanical heterogeneity that characterizes this syndrome.

Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury. Publishing Authors By Initials

BA Simon,RB Easley,DN Grigoryev,SF Ma,SQ Ye,T Lavoie,RM Tuder,JG Garcia,

For similar tomography, x-ray computed research abstracts see: tomography, x-ray computed research

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Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of physiology. Lung cellular and

VOLUME: 291

Page Numbers: L851-61

Journal Abbreviation: Am. J. Physiol. Lung Cell Mol.

ISSN: 1040-0605

DAY: 16

MONTH: 06

YEAR: 2006

Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury. Information

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LANGUAGE: eng

NlmUniqueID: 100901229

Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury. Keywords Mesh Terms:

KEYWORDS: Tomography, X-Ray Computed

MESH TERMS: radiography

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Grant and Affiliation Information for Microarray analysis of regional cellular responses to local mechanical stress in acute lung injury.

AFFILIATION: Department of Anesthesiology and Critical Medicine, Tower 711, Johns Hopkins Hospital, Baltimore, MD 21287-8711, USA. bsimon@jhmi.edu

Country: United States

AGENCY: United States NHLBI

GRANT: U01HL66583

ACRONYM: HL

MEDLINETA: Am J Physiol Lung Cell Mol Phy

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