Microarray analyses of newborn mouse ovaries lacking Nobox.

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Microarray analyses of newborn mouse ovaries lacking Nobox. Abstract Text:

Youngsok Choi,Yingying Qin,Michael F Berger,Daniel J Ballow,Martha L Bulyk,Aleksandar Rajkovic,Youngsok Choi,Yingying Qin,Michael F Berger,Daniel J Ballow,Martha L Bulyk,Aleksandar Rajkovic,

Nobox is a homeobox gene expressed in oocytes and critical in oogenesis. Nobox deficiency leads to rapid loss of postnatal oocytes. Early oocyte differentiation is poorly understood. We hypothesized that lack of Nobox perturbs global expression of genes preferentially expressed in oocytes as well as microRNAs. We compared Nobox knockout and wild-type ovaries using Affymetrix 430 2.0 microarray platform. We discovered that 28 (74%) of 38 of the genes downregulated more than 5-fold in the absence of Nobox were preferentially expressed in oocytes, whereas only 5 (15%) of 33 genes upregulated more than 5-fold in the absence of Nobox were preferentially expressed in oocytes. Protein-binding microarray helped identify nucleotide motifs that NOBOX binds and that several downregulated genes contain within putative promoter regions. MicroRNA population in newborn ovaries deficient of Nobox was largely unaffected. Genes whose proteins are predicted to be secreted but were previously unknown to be significantly expressed in early oogenesis were downregulated in Nobox knockouts and included astacin-like metalloendopeptidase (Astl), Jagged 1 (Jag1), oocyte-secreted protein 1 (Oosp1), fetuin beta (Fetub), and R-spondin 2 (Rspo2). In addition, pluripotency-associated genes Pou5f1 and Sall4 are drastically downregulated in Nobox-deficient ovaries, whereas testes-determining gene Dmrt1 is overexpressed. Our findings indicate that Nobox is likely an activator of oocyte-specific gene expression and suggest that the oocyte plays an important role in suppressing expression of male-determining genes, such as Dmrt1.

Microarray analyses of newborn mouse ovaries lacking Nobox. Publishing Authors By Initials

Y Choi,Y Qin,MF Berger,DJ Ballow,ML Bulyk,A Rajkovic,Y Choi,Y Qin,MF Berger,DJ Ballow,ML Bulyk,A Rajkovic,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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Microarray analyses of newborn mouse ovaries lacking Nobox. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biology of reproduction

VOLUME: 77

Page Numbers: 312-9

Journal Abbreviation: Biol. Reprod.

ISSN: 0006-3363

DAY: 9

MONTH: 05

YEAR: 2007

Microarray analyses of newborn mouse ovaries lacking Nobox. Information

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LANGUAGE: eng

NlmUniqueID: 207224

Microarray analyses of newborn mouse ovaries lacking Nobox. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: physiology

Chemical & Substance for Abstract: Microarray analyses of newborn mouse ovaries lacking Nobox. Information

Substance Name: Transcription Factors

Registry Number: 0

Grant and Affiliation Information for Microarray analyses of newborn mouse ovaries lacking Nobox.

AFFILIATION: Department of Obstetrics and Gynecology, Baylor College of Medicine, Houston, Texas 77030, USA.

Country: United States

AGENCY: United States NICHD

GRANT: HD044858

ACRONYM: HD

MEDLINETA: Biol Reprod

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