Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa.

Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Research Abstract Details 

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Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Abstract Text:

A Suzuki,S Handa,I Ishizuka,T Yamakawa,

Seminolipid (1-O-alkyl-2-O-acyl-beta-3'sulfogalactosyl glycerol) was detected on a micro-scale by high performance liquid chromatography (HPLC) using a UV detector. The benzoylation method of McCluer and Evans ((1973) J. Lipid Res. 14, 611-617) was used to convert seminolipid to a derivative with UV absorption. Total lipid from germinal tissue was benzoylated and the product was separated by Florisil and Sephadex LH-20 column chromatographies. The seminolipid obtained in the effluent from the Sephadex LH-20 column was determined by HPLC. Eighty percent recovery of 35S-labelled seminolipid was obtained by this method. The method was used to determine the seminolipid content of boar spermatozoa: 980 nmoles of seminolipid was found per gram of packed cells.

Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Publishing Authors By Initials

A Suzuki,S Handa,I Ishizuka,T Yamakawa,

For similar urogenital system: genitalia: genitalia, male: testis research abstracts see: urogenital system: genitalia: genitalia, male: testis research

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Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 81

Page Numbers: 127-34

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 1977

Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Keywords Mesh Terms:

KEYWORDS: Testis

MESH TERMS: metabolism

Chemical & Substance for Abstract: Micro-scale determination of seminolipid by high performance liquid chromatography and its application for determination of the seminolipid content in boar spermatozoa. Information

Substance Name: Sulfuric Acids

Registry Number: 0

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Country: JAPAN

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MEDLINETA: J Biochem

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