Micro-identification of amino-terminal acetylamino acids in proteins.

Micro-identification of amino-terminal acetylamino acids in proteins. Research Abstract Details 

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Micro-identification of amino-terminal acetylamino acids in proteins. Abstract Text:

S Tsunasawa,K Narita,

By using a reversed phase HPLC system, we have developed a rapid and quantitative micro-identification method for amino-terminal acetylamino acids in proteins at the sample range of 10--100 nmol. The process of the identification method consists of the following steps: (1) digestion of a protein by an appropriate protease; (2) separation of an acidic peptide(s) from the digest on a Dowex 50 x 2 column; (3) further purification of the acidic peptide(s) by reversed phase HPLC using a C18 column; (4) subsequent exhaustive digestion of each acidic peptide to amino acids and an acylamino acid, by pronase and carboxypeptidase Y; (5) isolation of the acylamino acid on a Dowex 50 x 2 column; (6) identification of the acylamino acid on a C18 column; (7) confirmation of the acyl group by HPLC as an 1-acyl-2-dansylhydrazine derivative and of the amino acid on amino acid analyzer. By applying this new method to such proteins as ovalbumin and cytochrome c, their amino-terminal acetylamino acids can be determined in the range of less than 10 nmol.

Micro-identification of amino-terminal acetylamino acids in proteins. Publishing Authors By Initials

S Tsunasawa,K Narita,

For similar proteins research abstracts see: proteins research

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Micro-identification of amino-terminal acetylamino acids in proteins. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 92

Page Numbers: 607-13

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1982

Micro-identification of amino-terminal acetylamino acids in proteins. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Micro-identification of amino-terminal acetylamino acids in proteins. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: methods

Chemical & Substance for Abstract: Micro-identification of amino-terminal acetylamino acids in proteins. Information

Substance Name: Peptide Hydrolases

Registry Number: EC 3.4.-

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Country: JAPAN

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MEDLINETA: J Biochem

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