Micro free-flow IEF enhanced by active cooling and functionalized gels.

Micro free-flow IEF enhanced by active cooling and functionalized gels. Research Abstract Details 

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Micro free-flow IEF enhanced by active cooling and functionalized gels. Abstract Text:

Jacob W Albrecht,Klavs F Jensen,

Rapid free-flow IEF is achieved in a microfluidic device by separating the electrodes from the focusing region with porous buffer regions. Moving the electrodes outside enables the use of large electric fields without the detrimental effects of bubble formation in the active region. The anode and cathode porous buffer regions, which are formed by acrylamide functionalized with immobilized pH groups, allow ion transport while providing buffering capacity. Thermoelectric cooling mitigates the effects of Joule heating on sample focusing at high field strengths (approximately 500 V/cm). This localized cooling was observed to increase device performance. Rapid focusing of low-molecular-weight p/ markers and Protein G-mouse IgG complexes demonstrate the versatility of the technique. Simulations provide insight into and predict device performance based on a well-defined sample composition.

Micro free-flow IEF enhanced by active cooling and functionalized gels. Publishing Authors By Initials

JW Albrecht,KF Jensen,

For similar proteins: nerve tissue proteins research abstracts see: proteins: nerve tissue proteins research

PUBMED ID PMID:

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Micro free-flow IEF enhanced by active cooling and functionalized gels. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Electrophoresis

VOLUME: 27

Page Numbers: 4960-9

Journal Abbreviation: Electrophoresis

ISSN: 0173-0835

DAY: 3

MONTH: Dec

YEAR: 2006

Micro free-flow IEF enhanced by active cooling and functionalized gels. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8204476

Micro free-flow IEF enhanced by active cooling and functionalized gels. Keywords Mesh Terms:

KEYWORDS: Nerve Tissue Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Micro free-flow IEF enhanced by active cooling and functionalized gels. Information

Substance Name: polyacrylamide gels

Registry Number: 0

Grant and Affiliation Information for Micro free-flow IEF enhanced by active cooling and functionalized gels.

AFFILIATION: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.

Country: Germany

AGENCY: United States NIGMS

GRANT: P50-GM68762

ACRONYM: GM

MEDLINETA: Electrophoresis

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