Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials.

Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Research Abstract Details 

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Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Abstract Text:

Xuesi M Shao,Jack L Feldman,

Maintaining a stable electrode potential is critical for patch-clamp measurements. The electrode potential of conventional patch electrode-holder assembly, where an Ag/AgCl wire is in direct contact with the patch pipette filling solution, is subject to drift if the pipette solution contains a low concentration of chloride ions (Cl-). We developed an agar bridge of 3 M KCl filled in a polyimide microtubing which forms an electrical connection between an Ag/AgCl wire and the pipette solution. We examined the offset potentials of the micro-agar salt bridge electrode assembly in parallel with a conventional electrode assembly in generic recording conditions (the pipette solution contained 5 mM NaCl). The junction potential between the Ag/AgCl wire and the pipette filling solution in the conventional electrode contributed to most of the offset potential drift observed during the course of 30 min recordings. The drift was up to 27.3 mV after several changes of the glass pipette. In contrast, the micro-agar salt bridge stabilized the electrode potential within typically 2 mV without affecting the patch electrode resistance, capacitance or noise level. Numerical simulations showed that Cl- diffusion from the agar bridge to the tip caused a negligible 0.4 microM Cl- concentration change at the pipette tip within 30 min. This method is easy to implement and provides long-term recording stability. The micro-agar salt bridge can fit in most commercial patch electrode holders and can be conveniently maintained.

Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Publishing Authors By Initials

XM Shao,JL Feldman,

For similar chlorides: potassium chloride research abstracts see: chlorides: potassium chloride research

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Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of neuroscience methods

VOLUME: 159

Page Numbers: 108-15

Journal Abbreviation: J. Neurosci. Methods

ISSN: 0165-0270

DAY: 17

MONTH: 08

YEAR: 2006

Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7905558

Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Keywords Mesh Terms:

KEYWORDS: Potassium Chloride

MESH TERMS: chemistry

Chemical & Substance for Abstract: Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials. Information

Substance Name: Agar

Registry Number: 9002-18-0

Grant and Affiliation Information for Micro-agar salt bridge in patch-clamp electrode holder stabilizes electrode potentials.

AFFILIATION: Department of Neurobiology, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095-1763, USA. mshao@ucla.edu

Country: Netherlands

AGENCY: United States NHLBI

GRANT: HL40959

ACRONYM: HL

MEDLINETA: J Neurosci Methods

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