Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells.

Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Research Abstract Details 

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Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Abstract Text:

Jenny J L Ho,Sang Won Han,Pamela L Pan,Guoren Deng,Shih-Fan Kuan,Young S Kim,

Anomalous expression of certain types of mucins occurs in pancreatic tumors, but little is known about the causes. MUC2 and MUC5AC are not expressed in normal pancreas. In the present study an immunohistochemical screening showed that MUC2 antigen was expressed in 6% of invasive tumors. Of the pancreatic cell lines, 2.4% expressed MUC2 message and antigen. In contrast, MUC5AC antigen was expressed in 86-100% of invasive adenocarcinoma (depending on the antibody). MUC5AC message and antigen were expressed in 66.7% of the cell lines tested. A polymerase chain reaction based assay was used to determine if methylation of CpG sites immediately 5' of the transcription initiation sites of the MUC2 and MUC5AC genes could be related to mucin expression in the cell lines. Digestibility by the methylation sensitive restriction enzyme HpaII correlated with the presence or absence of mRNA in 100% and 77.8% of the cell lines for MUC2 and MUC5AC, respectively. Treatment of a cell line that did not express MUC2 or MUC5AC gene products with the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine resulted in an increase in MUC2 message but no change in MUC5AC message. In summary, the expression of MUC2 gene products correlated well with methylation of the proximal region of the promoter whereas expression of MUC5AC may involve additional regions or other mechanisms.

Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Publishing Authors By Initials

JJ Ho,SW Han,PL Pan,G Deng,SF Kuan,YS Kim,

For similar nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, neoplasm research abstracts see: nucleic acids, nucleotides, and nucleosides: nucleic acids: rna: rna, neoplasm research

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MEDLINE DATE:

Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: International journal of oncology

VOLUME: 22

Page Numbers: 273-9

Journal Abbreviation: Int. J. Oncol.

ISSN: 1019-6439

DAY: 30

MONTH: Feb

YEAR: 2003

Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9306042

Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Keywords Mesh Terms:

KEYWORDS: RNA, Neoplasm

MESH TERMS: biosynthesis

Chemical & Substance for Abstract: Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells. Information

Substance Name: DNA (Cytosine-5-)-Methyltransferase

Registry Number: EC 2.1.1.37

Grant and Affiliation Information for Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells.

AFFILIATION: Gastrointestinal Research Laboratory (151 M2), Veterans Affairs Medical Center, San Francisco, CA 94121, USA. jjl@itsa.ucsf.edu

Country: Greece

AGENCY: United States NCI

GRANT: CA 24321

ACRONYM: CA

MEDLINETA: Int J Oncol

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