Method to enhance transfection efficiency of cell lines and placental fibroblasts.

Method to enhance transfection efficiency of cell lines and placental fibroblasts. Research Abstract Details 

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Method to enhance transfection efficiency of cell lines and placental fibroblasts. Abstract Text:

M Zhang,S Guller,Y Huang,

We report a method that allows for a 2-9-fold increase in transfection efficiency compared to the standard cationic lipid-based protocol. The method involves a brief incubation of freshly trypsinized cells with transfection complexes, followed by incubation in cell growth medium containing serum and antibiotics. The method is simple, cost-effective, and can be applied to both DNA and siRNA transfections as well as to a variety of cell types including hard-to-transfect human placental fibroblasts.

Method to enhance transfection efficiency of cell lines and placental fibroblasts. Publishing Authors By Initials

M Zhang,S Guller,Y Huang,

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PUBMED ID PMID:

MEDLINE DATE: 2007 Aug-Sep

Method to enhance transfection efficiency of cell lines and placental fibroblasts. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Placenta

VOLUME: 28

Page Numbers: 779-82

Journal Abbreviation: Placenta

ISSN: 0143-4004

DAY: 12

MONTH: 03

YEAR: 2007

Method to enhance transfection efficiency of cell lines and placental fibroblasts. Information

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LANGUAGE: eng

NlmUniqueID: 8006349

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Grant and Affiliation Information for Method to enhance transfection efficiency of cell lines and placental fibroblasts.

AFFILIATION: Department of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, 300 George Street, Suite 770, Room 759F, New Haven, CT 06511, USA.

Country: England

AGENCY: United States NICHD

GRANT: HD33909

ACRONYM: HD

MEDLINETA: Placenta

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