Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons.

Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons. Research Abstract Details 

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Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons. Abstract Text:

Vidya V Iyer,Ganesh Sriram,D Bruce Fulton,Ruilian Zhou,Mark E Westgate,Jacqueline V Shanks,

Metabolic flux maps developed from (13)C metabolic flux analysis ((13)C MFA) are effective tools for assessing the response of biological systems to genetic or environmental perturbations, and for identifying possible metabolic engineering targets. Experimental treatments were designed to distinguish between temperature effects prior to, and during incubation in vitro, on primary metabolism in developing soybeans. Biomass accumulation increased with temperature as did carbon partitioning into lipids. The flux through the plastidic oxidative pentose phosphate pathway (pgl(P)) relative to sucrose intake remained fairly constant [ approximately 56% (+/-24%)] when cotyledons were transferred from an optimum growth temperature to varying temperatures in in vitro culture, signifying a rigid node under these conditions. However, pgl(P) flux ranged from 57 to 77% of sucrose intake when growth temperature in planta varied and were cultured in vitro at the same temperature (as the plant), indicating a flexible node for this case. The carbon flux through the anaplerotic reactions catalysed by plastidic malic enzyme (me(P)), cytosolic phosphoenolpyruvate (PEP) carboxylase and the malate (Mal) transporter from the cytosol to mitochondrion varied dramatically with temperature and had a direct influence on the carbon partitioning into protein and oil from the plastidic pyruvate (Pyr) pool. These results of the in vitro culture indicate that temperature during early stages of development has a dominant effect on establishing capacity for flux through certain components of central carbon metabolism.

Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons. Publishing Authors By Initials

VV Iyer,G Sriram,DB Fulton,R Zhou,ME Westgate,JV Shanks,

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Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant, cell & environment

VOLUME: 31

Page Numbers: 506-17

Journal Abbreviation: Plant Cell Environ.

ISSN: 0140-7791

DAY: 9

MONTH: 01

YEAR: 2008

Metabolic flux maps comparing the effect of temperature on protein and oil biosynthesis in developing soybean cotyledons. Information

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LANGUAGE: eng

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AFFILIATION: Department of Chemical and Biological Engineering, 3031 Sweeney Hall, Iowa State University, Ames, IA 50011, USA.

Country: United States

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MEDLINETA: Plant Cell Environ

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