Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger.

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Abstract Text:

R Gheshlaghi,J M Scharer,M Moo-Young,P L Douglas,R Gheshlaghi,J M Scharer,M Moo-Young,P L Douglas,R Gheshlaghi,J M Scharer,M Moo-Young,P L Douglas,

A comprehensive metabolic network comprising three intracellular compartments (cytoplasm, mitochondrion and peroxisome) was developed for Aspergillus niger. The metabolic flux network includes carbohydrate and amino acid metabolism in both anabolic and catabolic reactions. Linear programming was used for the optimization of the specific growth rates in combination with 37 measured input and output fluxes of the key metabolites to evaluate corresponding intracellular flux distributions throughout the batch fermentations. Logarithmic sensitivity analysis revealed that the addition of proline, alanine and glutamate benefited growth in defined media. The experimental observations and flux analysis showed that tyrosine was a potential candidate for biomass production improvement. Model predictions was verified by conducting batch and fed-batch fermentations and it was found that the addition of the four amino acids according to the predetermined schedule resulted in a 44 and 41% improvements in biomass and recombinant protein productions, respectively.

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Publishing Authors By Initials

R Gheshlaghi,JM Scharer,M Moo-Young,PL Douglas,R Gheshlaghi,JM Scharer,M Moo-Young,PL Douglas,R Gheshlaghi,JM Scharer,M Moo-Young,PL Douglas,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Bioprocess and biosystems engineering

VOLUME: 30

Page Numbers: 397-418

Journal Abbreviation:

ISSN: 1615-7591

DAY: 16

MONTH: 07

YEAR: 2007

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101088505

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger.

AFFILIATION: Department of Chemical Engineering, University of waterloo, 200 University Avenue West, Waterloo, ON, Canada, N2L 3G1, rgheshla@engmail.uwaterloo.ca.

Country: Germany

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Bioprocess Biosyst Eng

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger Related Publications

• Adenoviral vectors for gene therapy.
• Alcohol dependence is independently associated with sepsis, septic shock, and hospital mortality among adult intensive care unit patients.
• Blastocyst-derived trophoblast stem cells from the rhesus monkey.
• Cell death, remodeling, and repair in chronic obstructive pulmonary disease?
• Consumer acceptable risk: how cigarette companies have responded to accusations that their products are defective.
• Control of ovulation in cycling mares with ovarian steroids and prostaglandin.
• Controlled ligand display on a symmetrical protein-cage architecture through mixed assembly.
• Converging epistemologies: critical issues in Canadian Inuit childbirth and pregnancy.
• Cross-linkage of hydroxyapatite/gelatin nanocomposite using imide-based zero-length cross-linker.
• Culture-negative Bartonella endocarditis in a patient with renal failure: the value of molecular methods in diagnosis.
• Frequency of insomnia report in patients with obstructive sleep apnoea hypopnea syndrome (OSAHS).
• Functional status in patients with narcolepsy.
• Fuzzy clustering of CT images for the measurement of hydrocephalus associated with tuberculous meningitis.
• High-density targeting of a viral multifunctional nanoplatform to a pathogenic, biofilm-forming bacterium.
• Left ventricular hypertrophy in athletes: morphologic features and clinical correlates.
• Lipid A fraction of LPS induces a discrete MAPK activation in acute lung injury.
• MUC1 is involved in trophoblast transendothelial migration.
• Measurement of plasma-derived substance P: biological, methodological, and statistical considerations.
• Metabolic flux analysis for optimizing the specific growth rate of recombinant Aspergillus niger.
• Plasma oxytocin concentrations in cyclic mares and sexually aroused stallions.
• Predictors of hepatitis B vaccination in women prisoners in two prisons in England.
• Preliminary findings of an intervention integrating modified directly observed therapy and risk reduction counseling.
• Relationship between the ability of oral streptococci to interact with platelet glycoprotein Ibalpha and with the salivary low-molecular-weight mucin, MG2.
• Sequence variation in alpha-methylacyl-CoA racemase and risk of early-onset and familial prostate cancer.
• Synthetic control over magnetic moment and exchange bias in all-oxide materials encapsulated within a spherical protein cage.
• Target-assembled ExciProbes: Application to DNA Detection at the Level of PCR Product and Plasmid DNA.
• Targeting of cancer cells with ferrimagnetic ferritin cage nanoparticles.
• The novel endocannabinoid receptor GPR55 is activated by atypical cannabinoids but does not mediate their vasodilator effects.
• The outcome of twin pregnancies in mares.
• The use of radioreceptor assay for the detection of pregnancy in the mare.