Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy.

Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy. Research Abstract Details 

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Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy. Abstract Text:

Robert S Allen,James A C Miller,Julie A Chitty,Anthony J Fist,Wayne L Gerlach,Philip J Larkin,Robert S Allen,James A C Miller,Julie A Chitty,Anthony J Fist,Wayne L Gerlach,Philip J Larkin,

We demonstrate that both over-expression and suppression of the gene encoding the morphinan pathway enzyme salutaridinol 7-O-acetyltransferase (SalAT) in opium poppy affects the alkaloid products that accumulate. Over-expression of the gene in most of the transgenic events resulted in an increase in capsule morphine, codeine and thebaine on a dry-weight basis. The transgenic line with the highest alkaloid content had 41%, 37% and 42% greater total alkaloids than the control in three independent trials over 3 years. DNA-encoded hairpin RNA-mediated suppression of SalAT resulted in the novel accumulation of the alkaloid salutaridine at up to 23% of total alkaloid; this alkaloid is not detectable in the parental genotype. Salutaridine is not the substrate of SalAT but the substrate of the previous enzyme in the pathway, salutaridine reductase. RNA transcript analysis of 16 primary T(0) transformants and their segregating T(1) progeny revealed an average reduction in SalAT transcript to about 12% of the control. Reduction in SalAT transcript was evident in both leaves and latex. Reverse transcriptase PCR and high-performance liquid chromatography analyses confirmed cosegregation of the expressed transgene with the salutaridine accumulating phenotype.

Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy. Publishing Authors By Initials

RS Allen,JA Miller,JA Chitty,AJ Fist,WL Gerlach,PJ Larkin,RS Allen,JA Miller,JA Chitty,AJ Fist,WL Gerlach,PJ Larkin,

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Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Plant biotechnology journal

VOLUME: 6

Page Numbers: 22-30

Journal Abbreviation: Plant Biotechnol. J.

ISSN: 1467-7652

DAY: 13

MONTH: 09

YEAR: 2007

Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Metabolic engineering of morphinan alkaloids by over-expression and RNAi suppression of salutaridinol 7-O-acetyltransferase in opium poppy.

AFFILIATION: CSIRO Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia.

Country: England

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MEDLINETA: Plant Biotechnol J

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