Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy.

Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Research Abstract Details 

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Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Abstract Text:

PURPOSE: To measure the levels of mRNA for genes important in cellular and extracellular matrix regulation in human corneal epithelium before and after photorefractive keratectomy (PRK) in order to explain myopic regression following surgery. METHODS: Scrapings from 26 normal corneas before a first photorefractive keratectomy were randomly pooled in two samples of 16 and 10 scraping, respectively, and compared to another 23 scrapings from corneas with myopic regression after a previous photorefractive keratectomy, also randomly pooled in another 2 samples of 16 and 7 scrapings each. The scrapings were analysed for seven different messenger RNAs involved in extracellular matrix using competition-based quantitative reverse-transcription polymerase chain reaction. RESULTS: Messenger RNAs for TGFalpha (Transforming growth factor-alpha), TGFbeta1 (Transforming growth factor-beta1), EGF-R (Epidermal growth factor-receptor) and TIMP1 (Tissue inhibitor metalloproteinase-1) were present in all samples. No mRNA for MMP9 (Metalloproteinase 9) or MMP2 (Metalloproteinase 2) were detected in any sample. Messenger RNA for collagen (alpha1) III was present in one sample following photorefractive keratectomy. CONCLUSIONS: The detection and measurement of levels of messenger RNA for selected growth factors, receptors, metalloproteinases and extracellular matrix proteins in ex vivo samples of human corneal epithelium is important and possible with a modified polymerase chain reaction technique. Messenger RNAs for Collagen III and for TGF-beta1 were elevated in one sample after photorefractive keratectomy.

Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Publishing Authors By Initials

For similar proteins: tissue inhibitor of metalloproteinases research abstracts see: proteins: tissue inhibitor of metalloproteinases research

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Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Acta ophthalmologica Scandinavica

VOLUME: 76

Page Numbers: 568-72

Journal Abbreviation: Acta Ophthalmol Scand

ISSN: 1395-3907

DAY: 17

MONTH: Oct

YEAR: 1998

Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Information

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LANGUAGE: eng

NlmUniqueID: 9507578

Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Keywords Mesh Terms:

KEYWORDS: Tissue Inhibitor of Metalloproteinases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy. Information

Substance Name: Receptor, Epidermal Growth Factor

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Messenger RNA levels for genes involved in extracellular matrix from human corneal scrapings before and after photorefractive keratectomy.

AFFILIATION: St. Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden.

Country: DENMARK

AGENCY: United States NEI

GRANT: EY05587

ACRONYM: EY

MEDLINETA: Acta Ophthalmol Scand

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