Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro.

Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Research Abstract Details 

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Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Abstract Text:

C Chang,D Niu,H Zhou,F Li,F Gong,C Chang,D Niu,H Zhou,F Li,F Gong,C Chang,D Niu,H Zhou,F Li,F Gong,

BACKGROUND: Extracellular microenvironment and intrinsic genetic programs determine the fate of stem cells. We observed whether mesenchymal stem cells (MSCs) contributed to insulin-producing cells in a manipulated microenvironment. METHODS: We delivered pancreatic pieces into Niobium-Coated Dynamatrix to construct a simulated pancreatic microenvironment, upon which soluble cytokine exchange and direct cell-cell contact between MSCs and pancreatic cells could occur. Bone marrow-derived MSCs were cultured upon the microenvironment. Differentiated isletlike cells were observed under an inverted microscope. Insulin in supernates was measurement by enzyme-linked immunosorbent assay. Insulin and c-peptide expression were verified by fluorescent immunocytochemistry and fluorescence in situ hybridization. Apoptosis of isletlike masses in high-glucose DMEM was detected by FACS. RESULTS: After 3 to 4 weeks in culture, typical isletlike masses were observed. Insulin secreted by differentiated cells (414.47+/-30.30 mIU/L) was much greater than that of undifferentiated cells (4.89+/-1.01 mIU/L; P<.05). Insulin and c-peptide expression were positive both in protein and mRNA levels. The transdifferentiated isletlike mass did not undergo apoptosis after another 3 weeks of culture in high-glucose DMEM. CONCLUSION: This simulated injury microenvironment without induction guided MSCs to functional isletlike cells hopefully to replace beta cells.

Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Publishing Authors By Initials

C Chang,D Niu,H Zhou,F Li,F Gong,C Chang,D Niu,H Zhou,F Li,F Gong,C Chang,D Niu,H Zhou,F Li,F Gong,

For similar animals: chordata: vertebrates: mammals: lagomorpha: rabbits research abstracts see: animals: chordata: vertebrates: mammals: lagomorpha: rabbits research

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Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Transplantation proceedings

VOLUME: 39

Page Numbers: 3363-8

Journal Abbreviation: Transplant. Proc.

ISSN: 0041-1345

DAY: 20

MONTH: Dec

YEAR: 2007

Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Information

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LANGUAGE: eng

NlmUniqueID: 243532

Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Keywords Mesh Terms:

KEYWORDS: Rabbits

MESH TERMS: cytology

Chemical & Substance for Abstract: Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro. Information

Substance Name: Insulin

Registry Number: 11061-68-0

Grant and Affiliation Information for Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro.

AFFILIATION: Department of Immunology Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Country: United States

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MEDLINETA: Transplant Proc

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