Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction.

Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Research Abstract Details 

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Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Abstract Text:

Vadim Cherezov,Martin Caffrey,

The membrane structural biologist seeks to understand how membrane proteins function at a molecular level. One of the most direct ways of accomplishing this requires knowing the structure of the protein, ideally at atomic resolution. To date, this can only be done by the method of macromolecular crystallography. Integral to the method is the need for three-dimensional crystals of diffraction quality and their production represents a major rate-limiting step in the overall process of structure determination. The in meso method is a novel approach for crystallizing membrane proteins. It makes use of lipidic mesophases, the cubic phase in particular. A mechanism for how the method works has been proposed. In this study, we set out to test one aspect of the hypothesis which posits that the protein migrates from the bulk mesophase reservoir to the face of the crystal by way of a lamellar conduit. Using a sub-micrometer-sized X-ray beam the interface between a growing membrane protein crystal and the bulk cubic phase was interrogated with micrometer spatial resolution. Characteristic diffraction from the lamellar phase was observed at the interface as expected. This result supports the proposal that the protein uses a lamellar portal on its way from the bulk mesophase up and into the face of the crystal.

Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Publishing Authors By Initials

V Cherezov,M Caffrey,

For similar proteins: membrane proteins research abstracts see: proteins: membrane proteins research

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Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Faraday discussions

VOLUME: 136

Page Numbers: 195-212; discussion 213-29

Journal Abbreviation: Faraday Discuss.

ISSN: 1359-6640

DAY: 28

MONTH: 11

YEAR: 2007

Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Information

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LANGUAGE: eng

NlmUniqueID: 9212301

Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Keywords Mesh Terms:

KEYWORDS: Membrane Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction. Information

Substance Name: Membrane Proteins

Registry Number: 0

Grant and Affiliation Information for Membrane protein crystallization in lipidic mesophases. A mechanism study using X-ray microdiffraction.

AFFILIATION: Department of Molecular Biology, The Scripps Research Institute, La Jolla CA 92037, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM61070

ACRONYM: GM

MEDLINETA: Faraday Discuss

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