Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin.

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Abstract Text:

D Caridha,D Yourick,M Cabezas,L Wolf,T H Hudson,G S Dow,D Caridha,D Yourick,M Cabezas,L Wolf,T H Hudson,G S Dow,

In previous studies, we have shown that mefloquine disrupts calcium homeostasis in neurons by depletion of endoplasmic reticulum (ER) stores, followed by an influx of external calcium across the plasma membrane. In this study, we explore two hypotheses concerning the mechanism(s) of action of mefloquine. First, we investigated the possibility that mefloquine activates non-N-methyl-d-aspartic acid receptors and the inositol phosphate 3 (IP3) signaling cascade leading to ER calcium release. Second, we compared the disruptive effects of mefloquine on calcium homeostasis to those of ionomycin in neuronal and nonneuronal cells. Ionomycin is known to discharge the ER calcium store (through an undefined mechanism), which induces capacitative calcium entry (CCE). In radioligand binding assays, mefloquine showed no affinity for the known binding sites of several glutamate receptor subtypes. The pattern of neuroprotection induced by a panel of glutamate receptor antagonists was dissimilar to that of mefloquine. Both mefloquine and ionomycin exhibited dose-related and qualitatively similar disruptions of calcium homeostasis in both neurons and macrophages. The influx of external calcium was blocked by the inhibitors of CCE in a dose-related fashion. Both mefloquine and ionomycin upregulated the IP3 pathway in a manner that we interpret to be secondary to CCE. Collectively, these data suggest that mefloquine does not activate glutamate receptors and that it disrupts calcium homeostasis in mammalian cells in a manner similar to that of ionomycin.

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Publishing Authors By Initials

D Caridha,D Yourick,M Cabezas,L Wolf,TH Hudson,GS Dow,D Caridha,D Yourick,M Cabezas,L Wolf,TH Hudson,GS Dow,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Antimicrobial agents and chemotherapy

VOLUME: 52

Page Numbers: 684-93

Journal Abbreviation: Antimicrob. Agents Chemother.

ISSN: 0066-4804

DAY: 12

MONTH: 11

YEAR: 2007

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 315061

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin.

AFFILIATION: Division of Experimental Therapeutics, Walter Reed Army Institute of Research, 503 Robert Grant Ave., Silver Spring, MD 20910. geoffrey.dow@na.amedd.army.mil.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Antimicrob Agents Chemother

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin Related Publications

• Action potentials of cerebellar cortex in response to local electrical stimulation.
• Carbon monoxide exposure on denali: comparing the 2004 and 2005 climbing seasons.
• Complementary global maps for orientation coding in upper and lower layers of the monkey's foveal striate cortex.
• Continuous low-dose oral chemotherapy for adjuvant therapy of splenic hemangiosarcoma in dogs.
• Corticotropin releasing hormone modulates endotoxin-induced inflammatory cytokine expression in human trophoblast cells.
• Diarrheal disease control studies; conical net for collecting flies.
• Diversification of the Function of Cell-to-Cell Signaling in Regulation of Virulence Within Plant Pathogenic Xanthomonads.
• EXTRINSIC REGULATORY MECHANISMS OF SEIZURE ACTIVITY.
• Evaluation of a novel tumor vaccine in dogs with hemangiosarcoma.
• Evaluation of a xenogeneic VEGF vaccine in dogs with soft tissue sarcoma.
• Excitability of skeletal muscle during development, denervation, and tissue culture.
• Foveal tracking cells in the superior temporal sulcus of the macaque monkey.
• Indications for steroid anaesthesia.
• Infants' early ability to segment the conversational speech signal predicts later language development: a retrospective analysis.
• Integrative physiology, functional genomics and the phenotype gap: a guide for comparative physiologists.
• Interspecies signalling via the Stenotrophomonas maltophilia diffusible signal factor influences biofilm formation and polymyxin tolerance in Pseudomonas aeruginosa.
• Intradural chordoma.
• Liposome-nucleic acid immunotherapeutics.
• Mefloquine-induced disruption of calcium homeostasis in Mammalian cells is similar to that induced by ionomycin.
• Neutrophil function in septic dogs.
• Oxidative stress during acute FIV infection in cats.
• Permanent transvenous dual-chamber pacing using the coronary sinus in a patient with a mechanical prosthetic tricuspid valve.
• Stance dependence of automatic postural adjustments in humans.
• Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
• The effect of prolonged veno-arterial pumping on the circulation of the dog.
• The establishment clause argument for choice.
• Treatment of cerebral palsy; clinical study in use of d-tubocurarine in peanut oil and myricin.
• Use of FoxP3 expression to identify regulatory T cells in healthy dogs and dogs with cancer.
• Use of radium in treatment of hypertrophic lymphoid tissue in the nasopharynx.
• Vaccination with liposome--DNA complexes elicits enhanced antitumor immunity.