MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome.

MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Research Abstract Details 

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MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Abstract Text:

Tatiana Nikitina,Rajarshi P Ghosh,Rachel A Horowitz-Scherer,Jeffrey C Hansen,Sergei A Grigoryev,Christopher L Woodcock,

hMeCP2 (human methylated DNA-binding protein 2), mutations of which cause most cases of Rett syndrome (RTT), is involved in the transmission of repressive epigenetic signals encoded by DNA methylation. The present work focuses on the modifications of chromatin architecture induced by MeCP2 and the effects of RTT-causing mutants. hMeCP2 binds to nucleosomes close to the linker DNA entry-exit site and protects approximately 11 bp of linker DNA from micrococcal nuclease. MeCP2 mutants differ in this property; the R106W mutant gives very little extra protection beyond the approximately 146-bp nucleosome core, whereas the large C-terminal truncation R294X reveals wild type behavior. Gel mobility assays show that linker DNA is essential for proper MeCP2 binding to nucleosomes, and electron microscopy visualization shows that the protein induces distinct conformational changes in the linker DNA. When bound to nucleosomes, MeCP2 is in close proximity to histone H3, which exits the nucleosome core close to the proposed MeCP2-binding site. These findings firmly establish nucleosomal linker DNA as a crucial binding partner of MeCP2 and show that different RTT-causing mutations of MeCP2 are correspondingly defective in different aspects of the interactions that alter chromatin architecture.

MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Publishing Authors By Initials

T Nikitina,RP Ghosh,RA Horowitz-Scherer,JC Hansen,SA Grigoryev,CL Woodcock,

For similar nervous system diseases: neurodegenerative diseases: heredodegenerative disorders, nervous system: rett syndrome research abstracts see: nervous system diseases: neurodegenerative diseases: heredodegenerative disorders, nervous system: rett syndrome research

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MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 28237-45

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 27

MONTH: 07

YEAR: 2007

MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Keywords Mesh Terms:

KEYWORDS: Rett Syndrome

MESH TERMS: metabolism

Chemical & Substance for Abstract: MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for MeCP2-chromatin interactions include the formation of chromatosome-like structures and are altered in mutations causing Rett syndrome.

AFFILIATION: Department of Biology, University of Massachusetts, Amherst, Massachusetts 01003, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM66834

ACRONYM: GM

MEDLINETA: J Biol Chem

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