Mechanisms underlying the radioprotective effect of histamine on small intestine.

Mechanisms underlying the radioprotective effect of histamine on small intestine. Research Abstract Details 

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Mechanisms underlying the radioprotective effect of histamine on small intestine. Abstract Text:

Vanina A Medina, Croci,Nora A Mohamad,Noelia Massari,Gloria Garbarino,Graciela P Cricco,Mariel A ,Gabriela A ,Ernesto J V Crescenti,Rosa M Bergoc,Elena S Rivera,Vanina A Medina, Croci,Nora A Mohamad,Noelia Massari,Gloria Garbarino,Graciela P Cricco,Mariel A ,Gabriela A ,Ernesto J V Crescenti,Rosa M Bergoc,Elena S Rivera,

PURPOSE: To examine the protective effects of histamine on intestinal damage produced by gamma-radiation. MATERIALS AND METHODS: 56 mice were divided into 4 groups. Histamine and Histamine-10 Gy groups received a daily subcutaneous histamine injection (0.1 mg/kg) starting 20 hours before irradiation and continued until the end of the experimental period; the untreated group received saline. Histamine-10 Gy and untreated-10 Gy groups were irradiated with a single dose on whole-body using Cesium-137 source (7 Gy/min) and were sacrificed 3 days after irradiation. Small intestine was removed, fixed and stained with hematoxylin and eosin. The number of intestinal crypts per circumference, and other histological characteristics of intestinal cells were evaluated. We further determined by immunohistochemistry the expression of proliferating cell nuclear antigen (PCNA), Bax, Bcl-2 (pro- and anti-apoptotic protein, respectively), antioxidant enzymes (Superoxide dismutase (SOD), Catalase and Glutathione peroxidase), histamine content and apoptosis by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labeling (TUNEL) assay. Cells in the S phase of the cell cycle were identified by immunohistochemical detection of 5-bromo-2'-deoxyuridine (BrdU) incorporation. RESULTS: Histamine treatment reduced mucosal atrophy, edema and preserved villi, crypts and nuclear and cytoplasmic characteristics of small intestine after radiation exposure. Additionally, histamine treatment increased PCNA expression and the BrdU-positive cell number, histamine content, decreased the number of apoptotic cells and significantly increased Catalase and copper-zinc-containing SOD of irradiated mice. CONCLUSIONS: Histamine prevents radiation-induced toxicity by increasing proliferation of damaged intestinal mucosa and suppressing apoptosis that was associated with an increase in SOD and Catalase levels. This effect might be of clinical value in patients undergoing radiotherapy.

Mechanisms underlying the radioprotective effect of histamine on small intestine. Publishing Authors By Initials

VA Medina,M Croci,NA Mohamad,N Massari,G Garbarino,GP Cricco,MA ,GA ,EJ Crescenti,RM Bergoc,ES Rivera,VA Medina,M Croci,NA Mohamad,N Massari,G Garbarino,GP Cricco,MA ,GA ,EJ Crescenti,RM Bergoc,ES Rivera,

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Mechanisms underlying the radioprotective effect of histamine on small intestine. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: International journal of radiation biology

VOLUME: 83

Page Numbers: 653-63

Journal Abbreviation: Int. J. Radiat. Biol.

ISSN: 0955-3002

DAY: 30

MONTH: Oct

YEAR: 2007

Mechanisms underlying the radioprotective effect of histamine on small intestine. Information

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LANGUAGE: eng

NlmUniqueID: 8809243

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AFFILIATION: Radioisotopes Laboratory, School of Pharmacy and Biochemistry, University of Buenos Aires.

Country: England

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MEDLINETA: Int J Radiat Biol

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