Mechanism of R-loop formation at immunoglobulin class switch sequences.

Mechanism of R-loop formation at immunoglobulin class switch sequences. Research Abstract Details 

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Mechanism of R-loop formation at immunoglobulin class switch sequences. Abstract Text:

Deepankar Roy,Kefei Yu,Michael R Lieber,Deepankar Roy,Kefei Yu,Michael R Lieber,Deepankar Roy,Kefei Yu,Michael R Lieber,

R-loops have been described in vivo at the immunoglobulin class switch sequences and at prokaryotic and mitochondrial origins of replication. However, the biochemical mechanism and determinants of R-loop formation are unclear. We find that R-loop formation is nearly eliminated when RNase T(1) is added during transcription but not when it is added afterward. Hence, rather than forming simply as an extension of the RNA-DNA hybrid of normal transcription, the RNA must exit the RNA polymerase and compete with the nontemplate DNA strand for an R-loop to form. R-loops persist even when transcription is done in Li(+) or Cs(+), which do not support G-quartet formation. Hence, R-loop formation does not rely on G-quartet formation. R-loop formation efficiency decreases as the number of switch repeats is decreased, although a very low level of R-loop formation occurs at even one 49-bp switch repeat. R-loop formation decreases sharply as G clustering is reduced, even when G density is kept constant. The critical level for R-loop formation is approximately the same point to which evolution drove the G clustering and G density on the nontemplate strand of mammalian switch regions. This provides an independent basis for concluding that the primary function of G clustering, in the context of high G density, is R-loop formation.

Mechanism of R-loop formation at immunoglobulin class switch sequences. Publishing Authors By Initials

D Roy,K Yu,MR Lieber,D Roy,K Yu,MR Lieber,D Roy,K Yu,MR Lieber,

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Mechanism of R-loop formation at immunoglobulin class switch sequences. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular and cellular biology

VOLUME: 28

Page Numbers: 50-60

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 1098-5549

DAY: 22

MONTH: 10

YEAR: 2007

Mechanism of R-loop formation at immunoglobulin class switch sequences. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Mechanism of R-loop formation at immunoglobulin class switch sequences.

AFFILIATION: USC Norris Comprehensive Cancer Ctr., Rm. 5428, 1441 Eastlake Ave., MC9176, Los Angeles, CA 90089-9176, USA.

Country: United States

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MEDLINETA: Mol Cell Biol

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