Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion.

Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Research Abstract Details 

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Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Abstract Text:

J Burda,M Chavko,

We have used brain (dog, rat) and spinal cord (dog, rabbit) cell-free systems to study early postischaemic inhibition of protein synthesis. Ischaemia alone produced a relatively small decrease in activity of all subcellular systems used. When 15 min of normoxic reperfusion was used, more than 30% decrease (p less than 0.01) in [14C]-leucine incorporation was detected. A translational inhibitor that appeared in the postribosomal supernatant fraction at the early stage of reperfusion reduced translational capacity of an initiating cell-free system. It also phosphorylated the small (38 kDa) subunit of eukaryotic initiation factor 2 (eIF-2) in vitro. Effect of the inhibitor can be reversed by addition of partially purified intact eIF-2 and/or high concentration (2 mmol/l) of GTP. A prevention of postischaemic free oxygen radical formation by the reoxygenation with hypoxaemic blood, containing 37.5 mm Hg O2 at 0-5 min and 56 mm Hg O2 at 6-10 min of recirculation, that was followed by 5 min of normoxic reperfusion, resulted in a significant increase (p less than 0.02) of polypeptide chain synthesis in vitro when compared with normoxic reperfusion.

Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Publishing Authors By Initials

J Burda,M Chavko,

For similar surgical procedures, operative: cardiovascular surgical procedures: reperfusion research abstracts see: surgical procedures, operative: cardiovascular surgical procedures: reperfusion research

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Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Physiological research / Academia Scientiarum Bohe

VOLUME: 40

Page Numbers: 395-402

Journal Abbreviation: Physiol Res

ISSN: 0862-8408

DAY: 2

MONTH: 04

YEAR: 1991

Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Information

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LANGUAGE: eng

NlmUniqueID: 9112413

Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Keywords Mesh Terms:

KEYWORDS: Reperfusion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion. Information

Substance Name: Oxygen

Registry Number: 7782-44-7

Grant and Affiliation Information for Mechanism of protein synthesis inhibition in CNS during postischaemic reperfusion.

AFFILIATION: Department of Neurochemistry, Slovak Academy of Sciences, Kosice, Czechoslovakia.

Country: CZECHOSLOVAKIA

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MEDLINETA: Physiol Res

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