Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver.

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Abstract Text:

K Sasaki,K Imamura,T Tanaka,

The effect of the ODC-factor, which was partially purified from ascites fluid of mice bearing Ehrlich ascites hepatoma, on DNA synthesis in the normal mouse liver and spleen was studied, and target cells for the factor in the liver were examined. DNA synthesis in the liver increased about 4-fold over the basal level 39-42 h after the increase of ODC activity induced by injection of the factor into normal mice. This increase of DNA synthesis was inhibited by repeated injection of DAPol. The inhibition was completely reversed by the administration of an appropriate amount of putrescine at about the same time. TK activity also increased in parallel with DNA synthesis. Normal mice with and without treatment with the factor were used to examine which cell population in the liver is the real target for the factor. The livers were dispersed and three cell populations (heavy, medium, and light) were separated by centrifugation. The heavy and light cell populations were characterized as mature hepatocytes and a cell population consisting mainly of immature hepatocytes and non-hepatocytes by analysis of marker enzymes, pyruvate kinase isozymes, L and M2, respectively. The factor stimulated ODC induction, with concomitant increases in TK and DNA poly activities and DNA synthesis, most effectively in the light cell fraction followed in order by the medium and heavy fractions. A nutritional factor (a high protein diet), which is a potent inducer of liver ODC, appeared to act on liver in a different way from the ODC-factor, judging from the results of studies of both whole liver and the fractionated cell system described above. Autoradiography of [3H]thymidine incorporation into liver cells showed that DNA synthesis in mature hepatocytes as well as nonhepatocytes was enhanced by injection of the factor. Stimulation of non-hepatocytes seems to be suggestive evidence that an immunologic response of mice might be developed by the factor. In fact, ODC activity, DNA synthesis, and DNA poly activity (but not TK) in the spleen significantly increased in response to the factor and their increments were suppressed by DAPol, though less sensitively than those in the light cell fraction of the liver.

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Publishing Authors By Initials

K Sasaki,K Imamura,T Tanaka,

For similar proteins research abstracts see: proteins research

PUBMED ID PMID:

MEDLINE DATE:

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 94

Page Numbers: 949-59

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Sep

YEAR: 1983

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver. Information

Substance Name: Ornithine Decarboxylase

Registry Number: EC 4.1.1.17

Grant and Affiliation Information for Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Mechanism of hepatic ornithine decarboxylase induction by the ornithine decarboxylase-inducing factor isolated from tumor ascites fluid: determination of target cells for the factor in the liver Related Publications

• A clonal analysis of neural progenitors during axolotl spinal cord regeneration reveals evidence for both spatially restricted and multipotent progenitors.
• Ankle skeletal injury predictions using anisotropic inelastic constitutive model of cortical bone taking into account damage evolution.
• Antiviral substances from the root of paeonia species.
• Application of a fine thread beam to the structure analysis of a hemihedrally twinned crystal of hydroxylamine oxidoreductase.
• Application of in vitro alternative methods to ecotoxicology.
• Association of Activities of Daily Living and Indices of Mental Status with Subsequent 20-year All-Cause Mortality in an Elderly Japanese Population.
• Circadian and light-induced transcription of clock gene Per1 depends on histone acetylation and deacetylation.
• Concentration of copper and a copper-EDTA complex at the pH junction formed in soil by an electrokinetic remediation process.
• Degradation of Ginseng Saponins under Mild Acidic Conditions.
• Diuretic action of paeonol.
• Effects Of Habitual Exercise And Gene Polymorphism On Quantitative Ultrasound Parameters In Japanese Elderly: 1291: Board #54 May 30 11:00 AM -12:30 PM.
• Ferritization treatment of copper in soil by electrokinetic remediation.
• Focal hepatic lesions mimicking cavernous hemangioma supplied by the portal vein.
• Further Study on Dammarane-Saponins of Leaves and Stems of American Ginseng, Panax quinquefolium.
• Improvement of the resonant cavity applicator for brain tumor hyperthermia - Computer simulation results -.
• Improvement of the resonant cavity applicator for brain tumor hyperthermia - Experimental heating results -.
• Influence of nutrient deficiency caused by host developmental arrest on the growth and development of a koinobiont parasitoid.
• Inhibition of NF-kappa B transcriptional activity by alpha-tocopheryl succinate.
• Optimal replication of poliovirus within cells.
• Panax ginseng: Relation between Age of Plant and Content of Ginsenosides.
• Proposal of a high-field superconducting wiggler for a slow positron source at SPring-8.
• Purification and Characterization of Antiviral Substance from the Bud of Syzygium aromatica.
• Seasonal variation of saponins, sucurose and monosaccharides in cultivated ginseng roots.
• Solubilization of saponins of bupleuri radix with ginseng saponins: cooperative effect of dammarane saponins.
• Studies on the Tissue Culture of Stevia rebaudiana and Its Components; (II)1. Induction of Shoot Primordia.
• Temperature distributions of developed needle type applicator.
• Tissue plasminogen activator and thrombin generation measurements using the Thrombinoscope.
• [Characteristics of polycyclic aromatic hydrocarbons (PAHS) on airborne particulates in Beijing]
• [Comparing identification of 3 kinds of flos chrysanthemi on surface morphology]
• [Drought tolerance of transgenic sweet potato expressing both Cu/Zn superoxide dismutase and ascorbate peroxidase]