Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents.

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Abstract Text:

Veera L D Badisa,Lekan M Latinwo,Caroline O Odewumi,Christopher O Ikediobi,Ramesh B Badisa,Lambert T Ayuk-Takem,Jude Nwoga,John West,

Cadmium is an environmental toxicant, which causes cancer in different organs. It was found that it damages DNA in the various tissues and cultured cell lines. To investigate the mechanism of DNA damage, we have studied the effect of cadmium-induced DNA damage in plasmid pBR322 DNA, and the possible ameliorative effects of antioxidative agents under in vitro conditions. It was observed that cadmium alone did not cause DNA damage. However, it caused DNA damage in the presence of hydrogen peroxide, in a dose dependent manner, because of production of hydroxyl radicals. Findings from this study show the conversion of covalently closed circular double-stranded pBR 322 DNA to the open circular and linear forms of DNA when treated with 10 muM cadmium and various concentrations of H(2)O(2). The conversion was due to nicking in DNA strands. The observed rate of DNA strand breakage was dependent on H(2)O(2) concentration, temperature, and time. Metallothionein I failed to prevent cadmium-induced DNA nicking in the presence of H(2)O(2). Of the two antioxidant enzymes (catalase and superoxide dismutase) studied, only catalase conferred significant (50-60%) protection. EDTA and DMSO exhibited protection similar to catalase, while mannitol showed only about 20% protection against DNA damage. Ethyl alcohol failed to ameliorate cadmium-induced DNA strands break. From this study, it is plausible to infer that cadmium in the presence of hydrogen peroxide causes DNA damage probably by the formation of hydroxyl ions. These results may indicate that cadmium in vivo could play a major role in the DNA damage induced by oxidative stress.

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Publishing Authors By Initials

VL Badisa,LM Latinwo,CO Odewumi,CO Ikediobi,RB Badisa,LT Ayuk-Takem,J Nwoga,J West,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

PUBMED ID PMID:

MEDLINE DATE:

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Environmental toxicology

VOLUME: 22

Page Numbers: 144-51

Journal Abbreviation: Environ. Toxicol.

ISSN: 1520-4081

DAY: 3

MONTH: Apr

YEAR: 2007

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100885357

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents. Information

Substance Name: Superoxide Dismutase

Registry Number: EC 1.15.1.1

Grant and Affiliation Information for Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents.

AFFILIATION: Department of Biology, Florida A&M University, Tallahassee, Florida 32307, USA.

Country: United States

AGENCY: United States PHS

GRANT: 36-1707-123

ACRONYM:

MEDLINETA: Environ Toxicol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents Related Publications

• A prospective, population-based study of the role of visual impairment in motor vehicle crashes among older drivers: the SEE study.
• A quantitative, theoretical framework for understanding mammalian sleep.
• American society of transplantation symposium on B cells in transplantation: harnessing humoral immunity from rodent models to clinical practice.
• Azithromycin for control of trachoma.
• Bioactive hydrogel substrates: probing leukocyte receptor-ligand interactions in parallel plate flow chamber studies.
• Causal inference for non-mortality outcomes in the presence of death.
• Changes in activity of the striatum during formation of a motor habit.
• Characterization of DNA-binding specificity and analysis of binding sites of the Pseudomonas aeruginosa global regulator, Vfr, a homologue of the Escherichia coli cAMP receptor protein.
• Defining the limits of conscientious objection in health care.
• Dopamine D2 receptor-dependent modulation of striatal NO synthase activity.
• Dynamic measures of visual function and their relationship to self-report of visual functioning.
• Endochondral bone formation from hydrogel carriers loaded with BMP2-transduced cells.
• Identifying factors which maximise the lambing performance of hoggets: a cross sectional study.
• Increased temporal and prefrontal activity in response to semantic associations in schizophrenia.
• Interaction of interleukin-6 and the BMP pathway in pulmonary smooth muscle.
• Lung cancer incidence in never smokers.
• Mechanism of DNA damage by cadmium and interplay of antioxidant enzymes and agents.
• Molecular effects of loss of BMPR2 signaling in smooth muscle in a transgenic mouse model of PAH.
• Morphometry of the extremely thin pulmonary blood-gas barrier in the chicken lung.
• On use of the multistage dose-response model for assessing laboratory animal carcinogenicity.
• Performance of lactating dairy cows fed whole cottonseed coated with gelatinized starch plus urea or yeast culture.
• Peritoneal dialysis: a primary care perspective.
• Poly(ethylene glycol) hydrogels conjugated with a collagenase-sensitive fluorogenic substrate to visualize collagenase activity during three-dimensional cell migration.
• Radiosensitivity, Radiogenomics & RAPPER.
• Scaling of number, size, and metabolic rate of cells with body size in mammals.
• Stabilization of RAD51 nucleoprotein filaments by the C-terminal region of BRCA2.
• The application of self-efficacy principles to audiologic rehabilitation: a tutorial.
• The prevalence of open-angle glaucoma among blacks and whites 73 years and older: the Salisbury Eye Evaluation Glaucoma Study.
• Variation in hospital admission for AMI unlikely to be attributable to smoking legislation.
• Young people who self-harm.