Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism.

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Abstract Text:

Cheng Fang,Melissa Behr,Fang Xie,Shijun Lu,Meghan Doret,Hongxiu Luo,Weizhu Yang,Kenneth Aldous,Xinxin Ding,Jun Gu,

Chloroform causes hepatic and renal toxicity in a number of species. In vitro studies have indicated that chloroform can be metabolized by P450 enzymes in the kidney to nephrotoxic intermediate, although direct in vivo evidence for the role of renal P450 in the nephrotoxicity has not been reported. This study was to determine whether chloroform renal toxicity persists in a mouse model with a liver-specific deletion of the P450 reductase (Cpr) gene (liver-Cpr-null). Chloroform-induced renal toxicity and chloroform tissue levels were compared between the liver-Cpr-null and wild-type mice at 24 h following differing doses of chloroform. At a chloroform dose of 150 mg/kg, the levels of blood urea nitrogen (BUN) were five times higher in the exposed group than in the vehicle-treated one for the liver-Cpr-null mice, but they were only slightly higher in the exposed group than in the vehicle-treated group for the wild-type mice. Severe lesions were found in the kidney of the liver-Cpr-null mice, while only mild lesions were found in the wild-type mice. At a chloroform dose of 300 mg/kg, severe kidney lesions were observed in both strains, yet the BUN levels were still higher in the liver-Cpr-null than in the wild-type mice. Higher chloroform levels were found in the tissues of the liver-Cpr-null mice. These findings indicated that loss of hepatic P450-dependent chloroform metabolism does not protect against chloroform-induced renal toxicity, suggesting that renal P450 enzymes play an essential role in chloroform renal toxicity.

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Publishing Authors By Initials

C Fang,M Behr,F Xie,S Lu,M Doret,H Luo,W Yang,K Aldous,X Ding,J Gu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Toxicology and applied pharmacology

VOLUME: 227

Page Numbers: 48-55

Journal Abbreviation: Toxicol. Appl. Pharmacol.

ISSN: 0041-008X

DAY: 23

MONTH: 10

YEAR: 2007

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 416575

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism.

AFFILIATION: Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509, USA; School of Public Health, State University of New York at Albany, NY 12201, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Toxicol Appl Pharmacol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Mechanism of chloroform-induced renal toxicity: Non-involvement of hepatic cytochrome P450-dependent metabolism Related Publications

• Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into porous coral in a nude mice model.
• [A discuss on risk factors of final height in 21-hydroxylase deficiency patients]
• [Assessment of sleep disorders in children with Tourette's syndrome]
• [Changes in the activities of enzymes involved in starch synthesis and accumulation in caryopsis of transgenic rice with antisense Wx gene]
• [Changes in the pulmonary function of factory workers exposure to terephthalic acid]
• [Clinical evaluation of two types of NiTi rotary instruments in preparation of root canals]
• [Comparative research on attenuation characteristics of human bladder cancer tissue at different wavelengths of laser and their linearly polarized laser in vitro]
• [Construction and expression in E. coli of single-chain F v fragment of antihuman activated platelets monoclonal antibody]
• [Culture and identification of human skin fibroblasts.]
• [Detection of IFN-gamma level in single CD8+ T cell]
• [Differentially expressed genes in hepatocellular carcinoma of tree shrew induced by different factors]
• [Effects of sodium nitroprusside on mitochondrial function of rye and wheat root tip under aluminum stress]
• [Human anti-HCMV neutralizing Fab antibody generated by phage display library]
• [IR and Raman spectroscopic studies on the complexes of ytterbium with pyran derivatives]
• [Induction of anti-tumor immunity by HSP gp96-peptide complexes]
• [Molecular genetic analysis of congenital lipoid adrenal hyperplasia]
• [Optical properties of human normal bladder tissue at five different wavelengths of laser and their linearly polarized laser irradiation in vitro]
• [Optical properties of human normal small intestine tissue with theoretical model of optics about biological tissues at Ar+ laser and 532 nm laser and their linearly polarized laser irradiation in vitro]
• [Prenatal diagnosis of glycogen storage disease Ia by screening for hot spot mutations in combination with the 1176 nucleotide polymorphism linkage analysis]
• [Production and some properties of a beta-glucanase from Trichoderma sp. GXC]
• [Purification and application of viral capsid antigen of Epstein-Barr virus]
• [Quantitative determination of insulin in microemulsion preparations by RP-HPLC]
• [Radial artery for coronary artery bypass grafting: operative techniques and early results]
• [Relation between pathologic tumor response to preoperative radiotherapy and the prognosis in patients with esophageal carcinoma]
• [Reporter genes transfer into human squamous cell carcinoma of larynx factor receptor mediated non-viral GE7 polypeptide delivery system with a new epidermal growth]
• [Research progress on the effect of zinc on behavior]
• [Solid phase extraction and room temperature phosphorimetry coupling technique and its application]
• [Study on the Raman spectra of ZYA system]
• [The applications of SERS to labeled immunoassay]
• [Value of intra-abdominal pressure monitoring in the prevention and treatment of multiple organ dysfunction syndrome]