[Mechanism of arsenic trioxide-induced apoptosis in hepatic blastoma cells HepG2]

[Mechanism of arsenic trioxide-induced apoptosis in hepatic blastoma cells HepG2] Research Abstract Details 

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[Mechanism of arsenic trioxide-induced apoptosis in hepatic blastoma cells HepG2] Abstract Text:

Ding Yu,Zi-hui Wang,Li-yuan Zhu,

OBJECTIVE: To investigate the mechanism of arsenic trioxide (As(2)O(3)) induced apoptosis in hepatic blastoma cells HepG2 and its effects on cell nuclear matrix related protein promyelocytic leukaemia (PML). METHODS: HepG2 cells were cultured in MEM medium and treated with different concentrations of As(2)O(3) for either 24 h or 96 h. In situ terminal deoxynucleotidyl transferase (TdT) labeling (TUNEL) and DNA ladder were applied to detect apoptosis. Confocal microscopy and western blot were performed to observe the expression of PML. RESULTS: TUNEL positive apoptotic cells and DNA ladder could be detected in As(2)O(3) treated groups. The expression of PML decreased in HepG2 cells with 2 micro mol/L As(2)O(3), and micropunctates characteristic of PML protein in HepG2 cell nuclei almost disappeared after the treatment of 5 micro mol/L As(2)O(3). CONCLUSION: As(2)O(3) induces HepG2 tumor cell apoptosis in a time- and concentration-dependent manner. As(2)O(3) may degradate the PML protein in HepG2 cell nuclei. The decreased expression of PML is closely correlated with apoptosis. Nuclear matrix associated protein PML could be the target of As(2)O(3) therapy.

[Mechanism of arsenic trioxide-induced apoptosis in hepatic blastoma cells HepG2] Publishing Authors By Initials

D Yu,ZH Wang,LY Zhu,

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[Mechanism of arsenic trioxide-induced apoptosis in hepatic blastoma cells HepG2] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua zhong liu za zhi [Chinese journal of onco

VOLUME: 25

Page Numbers: 120-3

Journal Abbreviation: Zhonghua Zhong Liu Za Zhi

ISSN: 0253-3766

DAY: 10

MONTH: Mar

YEAR: 2003

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LANGUAGE: chi

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AFFILIATION: Department of Surgery, Shenzhen Hospital, Beijing University, Shenzhen 518036, China.

Country: China

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MEDLINETA: Zhonghua Zhong Liu Za Zhi

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