Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair.

Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Research Abstract Details 

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Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Abstract Text:

Natalia Juncosa-Melvin,Karl S Matlin,Robert W Holdcraft,Victor S Nirmalanandhan,David L Butler,

Our group has shown that mechanical stimulation increases the stiffness of stem cell-collagen sponge constructs at 14 days in culture and subsequent rabbit patellar tendon repairs at 12 weeks postsurgery. What remains unclear is which genes might be responsible for this increase in stiffness. Therefore, the objective of this study was to determine how a tensile stimulus affects the gene expression of stem cell-collagen sponge constructs used to repair rabbit central patellar tendon defects. Tissue-engineered constructs were created by seeding mesenchymal stem cells (MSCs) from 10 adult rabbits at 0.14 x 10(6) cells/construct in type I collagen sponges. Half of the constructs were mechanically stimulated once every 5 min for 8 h/d to a peak strain of 2.4% for 2 weeks. The other half remained in an incubator without mechanical stimulation for 2 weeks. After 14 days in culture, half of the stimulated and nonstimulated constructs were prepared to determine the expression of collagen type I, collagen type III, decorin, fibronectin, and glyceraldehyde-3-phosphate dehydrogenase genes using real-time quantitative reverse transcriptase polymerase chain reaction. The remaining constructs were mechanically tested to determine their mechanical properties. Two weeks of in vitro mechanical stimulation significantly increased collagen type I and collagen type III gene expression of the stem cell-collagen sponge constructs. Stimulated constructs showed 3 and 4 times greater collagen type I (p = 0.0001) and collagen type III gene expression (p = 0.001) than nonstimulated controls. Stimulated constructs also had 2.5 times the linear stiffness and 4 times the linear modulus of nonstimulated constructs. However, mechanical stimulation did not significantly increase decorin or fibronectin gene expression (p = 0.2) after 14 days in culture. This study shows that mechanical stimulation of cell-sponge constructs produces similar increases in the expression of 2 structural genes, as well as linear stiffness and linear modulus.

Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Publishing Authors By Initials

N Juncosa-Melvin,KS Matlin,RW Holdcraft,VS Nirmalanandhan,DL Butler,

For similar biomechanics: tensile strength research abstracts see: biomechanics: tensile strength research

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Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Tissue engineering

VOLUME: 13

Page Numbers: 1219-26

Journal Abbreviation: Tissue Eng.

ISSN: 1076-3279

DAY: 3

MONTH: Jun

YEAR: 2007

Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Information

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LANGUAGE: eng

NlmUniqueID: 9505538

Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Keywords Mesh Terms:

KEYWORDS: Tensile Strength

MESH TERMS: surgery

Chemical & Substance for Abstract: Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair. Information

Substance Name: Collagen Type II

Registry Number: 0

Grant and Affiliation Information for Mechanical stimulation increases collagen type I and collagen type III gene expression of stem cell-collagen sponge constructs for patellar tendon repair.

AFFILIATION: Department of Biomedical Engineering, University of Cincinnati, Cincinnati, Ohio 45221-0048, USA. juncosln@email.uc.edu

Country: United States

AGENCY: United States NIAMS

GRANT: AR 46574

ACRONYM: AR

MEDLINETA: Tissue Eng

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