Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay.

Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay. Research Abstract Details 

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Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay. Abstract Text:

E Cook,X Wang,N Robiou,B C Fries,E Cook,X Wang,N Robiou,B C Fries,

Staphylococcal enterotoxin B (SEB) is a select agent because it is a potent mitogen that elicits life-threatening polyclonal T-cell proliferation and cytokine production at very low concentrations. Efforts are in progress to develop therapeutic reagents and vaccines that neutralize or prevent the devastating effects of this toxin. Because of its rapid binding to in vivo receptors, this toxin is difficult to detect in serum. This rapid binding also constitutes a major challenge for the development of effective therapeutic reagents that can neutralize the effects of the toxin in vivo. We have developed a highly sensitive capture enzyme-linked immunosorbent assay that detects SEB in body fluids at very low levels. With this assay, the peak levels of SEB in serum and renal clearance can be measured in mice. After either oral ingestion or nasal inhalation of SEB by mice, this assay documents the transcytosis of SEB across the mucosal membranes into serum within 2 h. Furthermore, this assay was used to compare the SEB levels in different murine models for SEB-induced lethal shock and demonstrated that the coadministration of toxin-enhancing chemicals, such as D-galactosamine and lipopolysaccharide, can alter the peak serum SEB levels. Hence, this assay is a potentially useful tool for the study of the pharmacokinetics of SEB and the effects of potential therapeutic reagents on serum SEB levels.

Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay. Publishing Authors By Initials

E Cook,X Wang,N Robiou,BC Fries,E Cook,X Wang,N Robiou,BC Fries,

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Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Clinical and vaccine immunology : CVI

VOLUME: 14

Page Numbers: 1094-101

Journal Abbreviation: Clin. Vaccine Immunol.

ISSN: 1556-6811

DAY: 18

MONTH: 07

YEAR: 2007

Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Measurement of staphylococcal enterotoxin B in serum and culture supernatant with a capture enzyme-linked immunosorbent assay.

AFFILIATION: Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park, Bronx, NY 10461, USA.

Country: United States

AGENCY: United States NIAID

GRANT: U54-AI057158

ACRONYM: AI

MEDLINETA: Clin Vaccine Immunol

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