Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography.

Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Abstract Text:

Jay H Williams,Stacey E Vidt,Janet Rinehart,Jay H Williams,Stacey E Vidt,Janet Rinehart,

The goal of this investigation was to develop an assay whereby we could measure changes in ATP, ADP, and phosphocreatine (PCr) during stimulation of the sarcoplasmic reticulum (SR) Ca(2+) ATPase. After stopping the enzyme reaction, compounds were extracted by perchloric acid and separated by reversed-phase high-performance liquid chromatography (HPLC). Absorbance of ATP and ADP was monitored at 260 nm, and detection of PCr was done at 205 nm. Chromatograms show that peaks associated with each compound are clearly separated and easily detected. The SR Ca(2+) ATPase assay was run for various time periods and using varying free [Ca(2+)]. The changes in ATP and ADP contents were linear with increasing time and varied as expected with increasing free [Ca(2+)]. The ATPase activities determined using changes in ATP and ADP were nearly identical to those determined using previously established assays. When PCr was added to the assay, we were able to confirm that the Ca(2+) ATPase uses ATP that is synthesized locally from PCr via creatine kinase (CK). The results indicate that this is a valid and reliable method for examining SR Ca(2+) ATPase activity and for investigating its interaction with CK.

Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Publishing Authors By Initials

JH Williams,SE Vidt,J Rinehart,JH Williams,SE Vidt,J Rinehart,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytical biochemistry

VOLUME: 372

Page Numbers: 135-9

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 25

MONTH: 09

YEAR: 2007

Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography.

AFFILIATION: Department of Human Nutrition, Foods, and Exercise, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Measurement of sarcoplasmic reticulum Ca2+ ATPase activity using high-performance liquid chromatography Related Publications

• A morphologic study of adrenal glands with correlations of body size and heart size.
• Analysis of the cardiovascular action of ibogaine hydrochloride.
• Capillary Resistance.
• Fronto-parietal and cerebellar contributions to motor dysfunction in Williams syndrome: A review and future directions.
• Measurement of sarcoplasmic reticulum Ca(2+) ATPase activity using high-performance liquid chromatography.
• Nebulized formoterol fumarate: Dose selection and pharmacokinetics.
• Observations on the Treatment of Rheumatic Fever with Vitamin P.
• Recurrent implantation failure: definition.
• Reduction of respirable silica following the introduction of water spray applications in Indian stone crusher mills.
• Rheumatic Fever and nutrition.
• Studies on the blood pyridoxine of vitamin B6 deficient monkeys.
• The epidemiology of cocaine and opiate abuse in urban Canada.
• Xanthurenic acid excretion in pyridoxine deficient rhesus monkeys.