Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays.

Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Research Abstract Details 

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Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Abstract Text:

Four monoclonal antibodies, MA-7D4, MA-7F5, MA-12A4 and MA-15H12, were raised against human plasminogen activator inhibitor-1 (PAI-1). MA-7D4 and MA-7F5 had an inhibitory effect on PAI-1 activity, whereas MA-15H12 and MA-12A4 did not affect PAI-1 activity. MA-7D4 was used previously as capture antibody in combination with MA-7F5 conjugated to horseradish peroxidase (7F5-HRP) to construct an ELISA which was 12 times more sensitive towards free PAI-1 as compared to PAI-1 in complex with human tissue-type plasminogen activator (t-PA) (Blood 71: 220-225, 1988). MA-15H12, as capture antibody, in combination with MA-12A4 corjugated to horseradish peroxidase (12A4-HRP) yielded an ELISA which was equally sensitive towards free PAI-1 and PAI-1/t-PA complex. Another ELISA was constructed using MA-15H12 as capture antibody in combination with an anti-t-PA antibody (MA-62E8) conjugated to horseradish peroxidase (62E8-HRP), allowing the specific measurement of t-PA/PAI-1 complexes and the development of an immunofunctional method for the specific quantitation of active PAI-1. These assays were then used to measure PAI-1 levels in plasma and the values obtained were compared with the data obtained by functional methods. It was found that 44% to 60% of free PAI-1 antigen in normal plasma is active and that t-PA occurs mainly as t-PA/PAI-1 complexes. In plasma from 48 patients suffering from angina pectoris no statistical difference in PAI-1 activity, PAI-1 antigen or PAI-1/t-PA complex could be observed as compared to normal subjects.(ABSTRACT TRUNCATED AT 250 WORDS)

Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Publishing Authors By Initials

For similar proteins: serpins: plasminogen inactivators research abstracts see: proteins: serpins: plasminogen inactivators research

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Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Thrombosis research. Supplement

VOLUME: 10

Page Numbers: 3-9

Journal Abbreviation: Thromb. Res. Suppl.

ISSN: 0049-3848

DAY: 28

MONTH: 02

YEAR: 1990

Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Information

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LANGUAGE: eng

NlmUniqueID: 8309239

Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Keywords Mesh Terms:

KEYWORDS: Plasminogen Inactivators

MESH TERMS: blood

Chemical & Substance for Abstract: Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays. Information

Substance Name: Plasminogen Inactivators

Registry Number: 0

Grant and Affiliation Information for Measurement of plasminogen activator inhibitor 1 (PAI-1) in plasma with various monoclonal antibody-based enzyme-linked immunosorbent assays.

AFFILIATION: Center for Thrombosis and Vascular Research, K.U. Leuven, Belgium.

Country: UNITED STATES

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MEDLINETA: Thromb Res Suppl

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