MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2.

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Abstract Text:

Yetao Jin,Shelya X Zeng,Xiao-Xin Sun,Hunjoo Lee,Christine Blattner,Zhixiong Xiao,Hua Lu,Yetao Jin,Shelya X Zeng,Xiao-Xin Sun,Hunjoo Lee,Christine Blattner,Zhixiong Xiao,Hua Lu,

We have shown previously that MDM2 promotes the degradation of the cyclin-dependent kinase inhibitor p21 through a ubiquitin-independent proteolytic pathway. Here we report that the MDM2 analog, MDMX, also displays a similar activity. MDMX directly bound to p21 and mediated its proteasomal degradation. Although the MDMX effect was independent of MDM2, they synergistically promoted p21 degradation when coexpressed in cells. This degradation appears to be mediated by the 26S proteasome, as MDMX and p21 bound to S2, one of the subunits of the 19S component of the 26S proteasome, in vivo. Conversely, knockdown of MDMX induced the level of endogenous p21 proteins that no longer cofractionated with 26S proteasome, resulting in G(1) arrest. The level of p21 was low at early S phase but markedly induced by knocking down either MDMX or MDM2 in human cells. Ablation of p21 rescued the G(1) arrest caused by double depletion of MDM2 and MDMX in p53-null cells. These results demonstrate that MDMX and MDM2 independently and cooperatively regulate the proteasome-mediated degradation of p21 at the G(1) and early S phases.

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Publishing Authors By Initials

Y Jin,SX Zeng,XX Sun,H Lee,C Blattner,Z Xiao,H Lu,Y Jin,SX Zeng,XX Sun,H Lee,C Blattner,Z Xiao,H Lu,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Molecular and cellular biology

VOLUME: 28

Page Numbers: 1218-29

Journal Abbreviation: Mol. Cell. Biol.

ISSN: 1098-5549

DAY: 17

MONTH: 12

YEAR: 2007

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8109087

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2.

AFFILIATION: Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Dr., MS4053, Indianapolis, IN 46202, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA93614

ACRONYM: CA

MEDLINETA: Mol Cell Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2 Related Publications

• Educational diagnosis of self-management behaviors of parents with asthmatic children by triangulation based on PRECEDE-PROCEED model in Taiwan.
• Effect of transforming growth factor-beta(2) on extracellular matrix synthesis in bovine trabecular meshwork cells.
• Influence of abnormal cervical pap smear undertaken during pregnancy to the outcome of the pregnancy.
• WT1 downregulation during K562 cell differentiation and apoptosis induced by bufalin.
• [Analysis of three-dimensional microstructure of poly(lactide-co-glycolide) scaffolds made by mild heating under high pressure]
• [Changes in plasma levels of adrenomedullin and cyclic adenosine monophosphate and their interrelation in patients with chronic heart failure]
• [Clinical significance of serum troponin I in patients with acute left heart failure]
• [Cloning and expression of the gene of recombinant augmenter of liver regeneration in yeast]
• [Collection, processing and cryopreservation of placental cord blood hematopoietic stem cells]
• [Construction of pcDNA3.1AM and expression of adrenomedullin in mammalian cells]
• [Cytogenetic analysis on 1058 cases of acute nonlymphocytic leukemia]
• [Determination of domoic acid in water and aquatic animals by high performance liquid chromatography]
• [Diagnosing achondroplasia by single cell nested-PCR]
• [Different effects of acetylcholine on the action potential and force contraction in guinea pig atrial and ventricular myocardium]
• [Effect of tumor necrosis factor-alpha on differentiation of mesencephalic neural stem cells and proliferation of oligodendrocytes in the rat]
• [Heterogeneity of action potential and ion currents in the left ventricular myocytes of the rabbit]
• [Human leukocyte antigen mismatched hemopietic stem cell transplants for the treatment of leukemia]
• [Human peripheral blood monocyte derived dendritic cell culture and mature regulation]
• [Inhibition of proliferation and induction of apoptosis by simvastatin in K562 leukemic cell line]
• [MK-801 suppresses dynorphin A (1-17)-induced facilitation of nociceptive responses to formalin in rats]
• [Molecular cytogenetic detection of partial chromosome 13q trisomy and its relation with the clinical features of tortilcollis]
• [Non-Hodgkin's lymphoma in the lacrimal gland: report of one case]
• [Reactive oxygen species and mitochondrial KATP-sensitive channels mediated cardioprotection induced by TNF-alpha during hypoxia and reoxygenation]
• [Relationship between soluble Fas ligand levels and complications after allogeneic bone marrow transplantation]
• [Relationship of I/D polymorphism of angiotensin converting enzyme gene with hypertension in Xinjiang Kazakh isolated group]
• [Sequencing of the glyceraldehyde-3-phosphate dehydrogenase gene from Plasmodium falciparum strain FCC1/HN]
• [Study on defibrinogenasing enhancing the thrombolytic effect of urokinase on acute myocardial infarction]
• [Study on preparation of beta-cyclodextrin inclusion compound of volatile oil from Aucklandia lappa]
• [The determination of F-, Cl-, NO3- and SO(4)2- in the total suspended particles in air by ion chromatography]
• [Treatment of primary trauma in the brainstem: analysis of 126 cases]