Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion.

Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Research Abstract Details 

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Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Abstract Text:

Joshua R Mauney,Carl Kirker-Head,Lauren Abrahamson,Gloria Gronowicz,Vladimir Volloch,David L Kaplan,

Mesenchymal stem cells (MSCs) represent an attractive cell source for tissue engineering applications, since they are readily isolated from adult bone marrow and have the ability to differentiate along multiple mesenchymal lineages, including osteogenic. Currently, utilization of MSCs for bone tissue engineering is limited because of the attenuation of their osteogenic differentiation potential and in vivo bone-forming capacity following ex vivo expansion on conventional tissue culture plastic (TCP). Previously, we demonstrated that a denatured type I collagen (DC) matrix promotes the maintenance of MSC in vitro osteogenic differentiation potential during ex vivo expansion in contrast to TCP. In this study, we further demonstrate that the maintenance of MSC osteogenic differentiation potential is primarily due to the ability of DC matrix to influence the retention of early passage osteogenic functions in late passage (LP) cells during ex vivo expansion, in contrast to solely enhancing attenuated LP cellular functions during osteogenic differentiation. Serum-associated factors played a significant role in influencing the retention of MSC osteogenic differentiation potential during expansion on the DC matrix. Significantly, the results show that although LP cells expanded ex vivo on TCP highly attentuate their in vivo bone-forming capacity, the expansion of MSCs on DC matrix preserves this ability as determined by histological, histomorphometric, and bone mineral density evaluations of MSC-seeded hydroxyapatite/tricalcium phosphate scaffolds following an 8-week implantation period within a heterotopic muscle pouch model. These findings provide further insight into the importance of matrix-mediated effects on MSC function and selective factors important in this process.

Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Publishing Authors By Initials

JR Mauney,C Kirker-Head,L Abrahamson,G Gronowicz,V Volloch,DL Kaplan,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein denaturation research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: protein denaturation research

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Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of biomedical materials research. Part A

VOLUME: 79

Page Numbers: 464-75

Journal Abbreviation:

ISSN: 1549-3296

DAY: 1

MONTH: Dec

YEAR: 2006

Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101234237

Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Keywords Mesh Terms:

KEYWORDS: Protein Denaturation

MESH TERMS: drug effects

Chemical & Substance for Abstract: Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion. Information

Substance Name: Collagen Type I

Registry Number: 0

Grant and Affiliation Information for Matrix-mediated retention of in vitro osteogenic differentiation potential and in vivo bone-forming capacity by human adult bone marrow-derived mesenchymal stem cells during ex vivo expansion.

AFFILIATION: Department of Biomedical Engineering and Chemical, Bioengineering and Biotechnology Center, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA.

Country: United States

AGENCY: United States NIBIB

GRANT: P41-EB002520

ACRONYM: EB

MEDLINETA: J Biomed Mater Res A

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