Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups.

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Abstract Text:

Jong-Seo Kim,Ji-Hyun Kim,Hie-Joon Kim,

Picolinamidination of amino groups in peptides was carried out using ethyl picolinimidate tetrafluoroborate synthesized from picolinamide and triethyloxonium tetrafluoroborate. The N-terminal amino group as well as the epsilon-amino group of lysine were derivatized. The matrix-assisted laser desorption/ionization (MALDI) signal of a peptide was enhanced 20-35-fold upon picolinamidination depending on the number of amino groups derivatized. The signal enhancement effect is much higher than that of acetamidination or guanidination previously reported. Improved protein identification by mass mapping of the derivatized peptides was demonstrated. Copyright (c) 2008 John Wiley & Sons, Ltd.

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Publishing Authors By Initials

JS Kim,JH Kim,HJ Kim,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Rapid communications in mass spectrometry : RCM

VOLUME: 22

Page Numbers: 495-502

Journal Abbreviation: Rapid Commun. Mass Spectrom.

ISSN: 0951-4198

DAY: 5

MONTH: 02

YEAR: 2008

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8802365

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups.

AFFILIATION: Department of Chemistry, Seoul National University, Seoul, Korea 151-747.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Rapid Commun Mass Spectrom

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Matrix-assisted laser desorption/ionization signal enhancement of peptides by picolinamidination of amino groups Related Publications

• Aberrant expression of MUC3 and MUC4 membrane-associated mucins and sialyl Le(x) antigen in pancreatic intraepithelial neoplasia.
• Aberrant expression of MUC5AC and MUC6 gastric mucins and sialyl Tn antigen in intraepithelial neoplasms of the pancreas.
• Advanced capabilities for future light sources.
• Aging of the population and the subsequent increase in the mistreatment of the elderly in Korea.
• Alkali-catalyzed beta-elimination of periodate-oxidized glycans: a novel method of chemical deglycosylation of mucin gene products in paraffin embedded sections.
• Altered mucin core peptide immunoreactivity in the colon polyp-carcinoma sequence.
• Basic fibroblast growth factor-induced translocation of p21-activated kinase to the membrane is independent of phospholipase C-gamma1 in the differentiation of PC12 cells.
• Biological properties and expression of mucins in 5-fluorouracil resistant HT29 human colon cancer cells.
• Cholangiocarcinomas arising in cirrhosis and combined hepatocellular-cholangiocellular carcinomas share apomucin profiles.
• Chromosomal clustering of muscle-expressed genes in Caenorhabditis elegans.
• Drynariae Rhizoma promotes osteoblast differentiation and mineralization in MC3T3-E1 cells through regulation of bone morphogenetic protein-2, alkaline phosphatase, type I collagen and collagenase-1.
• Elderly Women's Grief in Korea.
• Expression of MUC2, MUC5AC and MUC6 apomucins in carcinoma, dysplasia and non-dysplastic epithelia of the gallbladder.
• Goblet cell-specific expression mediated by the MUC2 mucin gene promoter in the intestine of transgenic mice.
• Identification and characterization of the MUC2 (human intestinal mucin) gene 5'-flanking region: promoter activity in cultured cells.
• Immunohistochemical study of mucin carbohydrates and core proteins in hepatolithiasis and cholangiocarcinoma.
• Induction of mucin gene expression in middle ear of rats by tumor necrosis factor-alpha: potential cause for mucoid otitis media.
• Initiation of transcription of the MUC3A human intestinal mucin from a TATA-less promoter and comparison with the MUC3B amino terminus.
• MUC3 human intestinal mucin. Analysis of gene structure, the carboxyl terminus, and a novel upstream repetitive region.
• Methylation status of promoters and expression of MUC2 and MUC5AC mucins in pancreatic cancer cells.
• Molecular cloning of human intestinal mucin (MUC2) cDNA. Identification of the amino terminus and overall sequence similarity to prepro-von Willebrand factor.
• Monoclonal antibodies against partially deglycosylated colon cancer mucin that recognize Tn antigen.
• Mouse intestinal goblet cells expressing SV40 T antigen directed by the MUC2 mucin gene promoter undergo apoptosis upon migration to the villi.
• Optical design of the U7 undulator beamline at the Pohang Light Source.
• Phorbol 12-myristate 13-acetate induces alteration in mucin gene expression and biological properties of colon cancer cells.
• Phorbol 12-myristate 13-acetate up-regulates the transcription of MUC2 intestinal mucin via Ras, ERK, and NF-kappa B.
• Stent-graft placement in a traumatic internal carotid-internal jugular fistula and pseudoaneurysm.
• Stereotactic aspiration of intracerebral haematoma: significance of surgical timing and haematoma volume reduction.
• Study of the aging perception.
• The Shank family of postsynaptic density proteins interacts with and promotes synaptic accumulation of the beta PIX guanine nucleotide exchange factor for Rac1 and Cdc42.