Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization.

Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Research Abstract Details 

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Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Abstract Text:

Jun Liu,Taocong Jin,Syweren Chang,Helena H Ritchie,Anthony J Smith,Brian H Clarkson,

We have recently reported the induction of dental pulp stem cells (DPSCs) into dentin-secreting odontoblast-like cells after stimulation by isolated dentin matrix components, thus mimicking the nature of tissue regeneration seen after tooth disease and injury. After confluency, the cells were further cultured for 21 d in the 10% fetal bovine serum (FBS) Dulbecco's modified Eagle's medium (DMEM) (control), and in this medium, with the addition of dentin extract (DE) and the mineralization supplement (MS) of ascorbic acid and beta-glycerophosphate (treatment). To identify genes associated with this process, specimens were analyzed with a HG-U133A human gene chip and Arrayassist software. A total of 425 genes, among them 21 matrix and eight TGF-beta-related genes, were either up- or downregulated in the experimental group in which the cells showed odontoblast-like differentiation and mineralization. Expression of selected genes was further confirmed by real-time polymerase chain reaction (PCR) analysis. Of the extracellular matrix (ECM)-related genes, two types of collagen genes were upregulated and seven others downregulated. Other ECM-related genes, for example fibulin-1, tenascin C, and particularly thrombospondin 1, were upregulated, and fibulin-2 was downregulated. Most noticeably, the matrix metalloproteinase 1 was induced by the treatment. In the TGF-beta superfamily, upregulation of the type II receptor, endoglin, and growth/differentiation factor 5 was coordinated with the downregulation of activin A, TGF-beta2, and TGF-beta1 itself. This study identifies the matrix and TGF-beta-related gene profiles during the DPSC cell mineralization in which several genes are reported for the first time to be associated with this process, thus greatly expanding our molecular knowledge of the induced disease repair process.

Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Publishing Authors By Initials

J Liu,T Jin,S Chang,HH Ritchie,AJ Smith,BH Clarkson,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: In vitro cellular & developmental biology. Animal

VOLUME: 43

Page Numbers: 120-8

Journal Abbreviation: In Vitro Cell. Dev. Biol. Anim

ISSN: 1071-2690

DAY: 22

MONTH: 05

YEAR: 2007

Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9418515

Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization. Information

Substance Name: beta-glycerophosphoric acid

Registry Number: 17181-54-3

Grant and Affiliation Information for Matrix and TGF-beta-related gene expression during human dental pulp stem cell (DPSC) mineralization.

AFFILIATION: Department of Cariology, Restorative Sciences and Endodontics, School of Dentistry, University of Michigan, 1011 North University, Ann Arbor, MI 48109-1078, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: DE12899

ACRONYM: DE

MEDLINETA: In Vitro Cell Dev Biol Anim

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