Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells.

Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells. Research Abstract Details 

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Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells. Abstract Text:

Tiejun Jiang,Yongliang Zhu,Cong Luo,Xiaoyong Lu,Wo Zhang,Fuming Qiu,Jian Huang,Tiejun Jiang,Yongliang Zhu,Cong Luo,Xiaoyong Lu,Wo Zhang,Fuming Qiu,Jian Huang,

Matrine, from Sophora flavescens, could remarkably inhibit tumor growth and induce apoptosis in various cancer cells in vitro. eIF4E and its inhibitor 4E-BP1 play key roles in regulating mRNA translation and cell proliferation. However, it remained elusive whether matrine inhibited cancer cells growth through attenuating the activity of 4E-BP1. In this study, we analyzed the effects of matrine on 4E-BP1 and eIF4E in gastric cancer MKN45 cells. Immunoblots showed that matrine inhibited the activity of eIF4E through dephosphorylation of 4E-BP1 in a dose- and time-dependent manner. We found that matrine inactivated Erk1/2, an upstream regulator of 4E-BP1 and eIF4E, and remarkably reduced the phosphorylation level of 4E-BP1 and eIF4E, whereas 4E-BP1 was little influenced by JNK, p38 or Akt/mTOR. Inactivation of PP2A obviously decreased the phosphorylation of 4E-BP1 in matrine-treated cells. These findings suggested that matrine inhibits the activity of eIF4E by dephosphorylating 4E-BP1, which partly counts for the growth inhibition in gastric MKN45 cells.

Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells. Publishing Authors By Initials

T Jiang,Y Zhu,C Luo,X Lu,W Zhang,F Qiu,J Huang,T Jiang,Y Zhu,C Luo,X Lu,W Zhang,F Qiu,J Huang,

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Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Planta medica

VOLUME: 73

Page Numbers: 1176-81

Journal Abbreviation: Planta Med.

ISSN: 0032-0943

DAY: 7

MONTH: 09

YEAR: 2007

Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells. Information

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LANGUAGE: eng

NlmUniqueID: 66751

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Grant and Affiliation Information for Matrine inhibits the activity of translation factor eIF4E through dephosphorylation of 4E-BP1 in gastric MKN45 cells.

AFFILIATION: Cancer Institute, Department of Oncology, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, P. R. China.

Country: Germany

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MEDLINETA: Planta Med

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