Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study.

Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Research Abstract Details 

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Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Abstract Text:

S Y Ohki,M Yazawa,K Yagi,K Hikichi,

The interaction between calmodulin and mastoparan at various concentrations of calcium ions was studied by 1H NMR. It was found that at lower mastoparan concentrations 1 mol of mastoparan binds to both the C-terminal-half and N-terminal-half regions of calcium-saturated calmodulin. The mastoparan affinity is much greater for the C-terminal-half region than for the N-terminal-half region. At higher mastoparan concentrations, a further 1 mol of mastoparan binds to the N-terminal-region of calcium saturated calmodulin. The results can be interpreted in terms of the assumption that the N-terminal-half region of calmodulin with mastoparan has a higher calcium ion affinity than the C-terminal-half region without mastoparan. It is suggested that calcium ions transfer from the C-terminal-half region of calmodulin without mastoparan to the N-terminal-half region of calmodulin with mastoparan. This calcium ion transfer is discussed from the viewpoint of enzyme activation by calmodulin.

Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Publishing Authors By Initials

SY Ohki,M Yazawa,K Yagi,K Hikichi,

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Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 110

Page Numbers: 737-42

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Nov

YEAR: 1991

Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Keywords Mesh Terms:

KEYWORDS: Wasp Venoms

MESH TERMS: chemistry

Chemical & Substance for Abstract: Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study. Information

Substance Name: Calcium

Registry Number: 7440-70-2

Grant and Affiliation Information for Mastoparan binding induces Ca(2+)-transfer between two globular domains of calmodulin: a 1H NMR study.

AFFILIATION: Department of Polymer Science, Faculty of Science, Hokkaido University.

Country: JAPAN

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MEDLINETA: J Biochem

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