Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase.

Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Research Abstract Details 

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Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Abstract Text:

Tatsuya Yamamoto,Shunsuke Izumi,Kunihiko Gekko,

To address the effects of local structures on structural fluctuations of Escherichia coli dihydrofolate reductase (DHFR), the backbone-fluctuation map was determined by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) coupled with H/D exchange and pepsin digestion. H/D exchange kinetics was examined at 15 degrees C with 18 identified digestion fragments covering almost the entire amino acid sequence of DHFR. These fragments exhibited significant variations in the first-order rate constant of proton exchange, k(ex) (0.47-0.71 min(-1)), the fraction of deuterium incorporation at the initial stage, D(o) (0.20-0.60), the fraction of deuterium incorporation at infinite time, D(infinity) (0.75-0.97), and the number of protons protected from exchange, P (0.4-4.7), relative to the corresponding values for the whole DHFR molecule (k(ex) = 0.51 min(-1), D(o) = 0.41, D(infinity) = 0.85, and P = 20.7). H/D exchange was very fast in the fragment comprising residues 5-28 (Met20 loop), which participates in substrate uptake, and reasonably fast in disordered and hydrophobic fragments, but slow in beta-strand-rich fragments. These results indicate that the local structures contribute differently to the fluctuation of the DHFR molecule, and that mass spectrometry coupled with H/D exchange and protease digestion is a useful tool for detecting segment-dependent protein fluctuation.

Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Publishing Authors By Initials

T Yamamoto,S Izumi,K Gekko,

For similar enzymes and coenzymes: enzymes: oxidoreductases: oxidoreductases acting on ch-nh group donors: tetrahydrofolate dehydrogenase research abstracts see: enzymes and coenzymes: enzymes: oxidoreductases: oxidoreductases acting on ch-nh group donors: tetrahydrofolate dehydrogenase research

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Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 135

Page Numbers: 17-24

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2004

Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Keywords Mesh Terms:

KEYWORDS: Tetrahydrofolate Dehydrogenase

MESH TERMS: genetics

Chemical & Substance for Abstract: Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase. Information

Substance Name: Tetrahydrofolate Dehydrogenase

Registry Number: EC 1.5.1.3

Grant and Affiliation Information for Mass spectrometry on segment-specific hydrogen exchange of dihydrofolate reductase.

AFFILIATION: Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526.

Country: Japan

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MEDLINETA: J Biochem

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