Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins.

Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Research Abstract Details 

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Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Abstract Text:

Lianmei Feng,Ron Orlando,James H Prestegard,

Application of nuclear magnetic resonance (NMR) methods for the structural characterization to larger and more complex protein systems can be facilitated through the development of new methods for resonance assignment. Here, a novel approach that relies on integration of NMR and mass spectrometry (MS) methods is explored. The approach relies on the fact that both NMR and MS are able to monitor rates of exchange of amide protons for water deuterons. Correlating the rates can connect cross-peak positions from NMR data with fragment masses from MS data to support sequential assignment. The example provided is for a small model protein, ubiquitin, but the potential for application to large, more difficult to express proteins is clear.

Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Publishing Authors By Initials

L Feng,R Orlando,JH Prestegard,

For similar proteins: ubiquitins: ubiquitin research abstracts see: proteins: ubiquitins: ubiquitin research

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Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 14377-9

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 10

MONTH: Nov

YEAR: 2004

Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Keywords Mesh Terms:

KEYWORDS: Ubiquitin

MESH TERMS: chemistry

Chemical & Substance for Abstract: Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins. Information

Substance Name: Ubiquitin

Registry Number: 0

Grant and Affiliation Information for Mass spectrometry assisted assignment of NMR resonances in 15N labeled proteins.

AFFILIATION: Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602, USA.

Country: United States

AGENCY: United States NCRR

GRANT: RR05351

ACRONYM: RR

MEDLINETA: J Am Chem Soc

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