Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus.

Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Research Abstract Details 

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Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Abstract Text:

Elizabeth A Stemmler,Emily A Bruns,Noah P Gardner,Patsy S Dickinson,Andrew E Christie,

In invertebrates, peptides possessing the carboxy (C)-terminal motif -RXRFamide have been proposed as the homologs of vertebrate neuropeptide Y (NPY). Using matrix assisted laser desorption/ionization mass spectrometry, in combination with sustained off-resonance irradiation collision-induced dissociation and chemical and enzymatic reactions, we have identified the peptide pEGFYSQRYamide from the neuroendocrine pericardial organ (PO) of the crab Pugettia producta. This peptide is likely the same as that previously reported, but misidentified, as PAFYSQRYamide in several earlier reports (e.g. [Li, L., Kelley, W.P., Billimoria, C.P., Christie, A.E., Pulver, S.R., Sweedler, J.V., Marder, E. 2003. Mass spectrometric investigation of the neuropeptide complement and release in the pericardial organs of the crab, Cancer borealis. J. Neurochem. 87, 642-656; Fu, Q., Kutz, K.K., Schmidt, J.J., Hsu, Y.W., Messinger, D.I., Cain, S.D., de la Iglesia, H.O., Christie, A.E., Li, L. 2005. Hormone complement of the Cancer productus sinus gland and pericardial organ: an anatomical and mass spectrometric investigation. J. Comp. Neurol. 493, 607-626.]). The -QRYamide motif contained in pEGFYSQRYamide is identical to that present in many vertebrate members of the NPY superfamily. Mass spectrometric analysis conducted on the POs of several other decapods showed that pEGFYSQRYamide is present in three other brachyurans (Cancer borealis, Cancer irroratus and Cancer productus) as well as in one species from another decapod infraorder (Lithodes maja, an anomuran). Thus, our findings show that at least some invertebrates possess NPY-like peptides in addition to those exhibiting an -RXRFamide C-terminus, and raise the question as to whether the invertebrate -QRYamides are functionally and/or evolutionarily related to the NPY superfamily.

Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Publishing Authors By Initials

EA Stemmler,EA Bruns,NP Gardner,PS Dickinson,AE Christie,

For similar hormones, hormone substitutes, and hormone antagonists: hormones: peptide hormones research abstracts see: hormones, hormone substitutes, and hormone antagonists: hormones: peptide hormones research

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Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: General and comparative endocrinology

VOLUME: 152

Page Numbers: 1-7

Journal Abbreviation:

ISSN: 0016-6480

DAY: 2

MONTH: 03

YEAR: 2007

Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Information

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LANGUAGE: eng

NlmUniqueID: 370735

Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Keywords Mesh Terms:

KEYWORDS: Peptide Hormones

MESH TERMS: metabolism

Chemical & Substance for Abstract: Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus. Information

Substance Name: Peptide Hormones

Registry Number: 0

Grant and Affiliation Information for Mass spectrometric identification of pEGFYSQRYamide: a crustacean peptide hormone possessing a vertebrate neuropeptide Y (NPY)-like carboxy-terminus.

AFFILIATION: Department of Chemistry, Bowdoin College, 6600 College Station, Brunswick, ME 04011, USA. estemmle@bowdoin.edu

Country: United States

AGENCY: United States NCRR

GRANT: P20RR-016463

ACRONYM: RR

MEDLINETA: Gen Comp Endocrinol

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