Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain.

Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain. Research Abstract Details 

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Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain. Abstract Text:

Tatsuhiko Sakamoto,Akiyoshi Uezu,Shinya Kawauchi,Takuya Kuramoto,Koji Makino,Kazuaki Umeda,Norie Araki,Hideo Baba,Hiroyuki Nakanishi,

Microtubules (MTs) play crucial roles in a variety of cell functions, such as mitosis, vesicle transport and cell motility. MTs also compose specialized structures, such as centrosomes, spindles and cilia. However, molecular mechanisms of these MT-based functions and structures are not fully understood. Here, we analyzed MT co-sedimented proteins from rat brain by tandem mass spectrometry (MS) upon ion exchange column chromatography. We identified a total of 391 proteins. These proteins were grouped into 12 categories: 57 MT cytoskeletal proteins, including MT-associated proteins (MAPs) and motor proteins; 66 other cytoskeletal proteins; 4 centrosomal proteins; 10 chaperons; 5 Golgi proteins; 7 mitochondrial proteins; 62 nucleic acid-binding proteins; 14 nuclear proteins; 13 ribosomal proteins; 28 vesicle transport proteins; 83 proteins with diverse function and/or localization; and 42 uncharacterized proteins. Of these uncharacterized proteins, six proteins were expressed in cultured cells, resulting in the identification of three novel components of centrosomes and cilia. Our present method is not specific for MAPs, but is useful for identifying low abundant novel MAPs and components of MT-based structures. Our analysis provides an extensive list of potential candidates for future study of the molecular mechanisms of MT-based functions and structures.

Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain. Publishing Authors By Initials

T Sakamoto,A Uezu,S Kawauchi,T Kuramoto,K Makino,K Umeda,N Araki,H Baba,H Nakanishi,

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Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genes to cells : devoted to molecular & cellular m

VOLUME: 13

Page Numbers: 295-312

Journal Abbreviation: Genes Cells

ISSN: 1365-2443

DAY: 26

MONTH: Apr

YEAR: 2008

Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain. Information

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LANGUAGE: eng

NlmUniqueID: 9607379

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Grant and Affiliation Information for Mass spectrometric analysis of microtubule co-sedimented proteins from rat brain.

AFFILIATION: Department of Molecular Pharmacology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860-8556, 1-1-1 Honjo, Kumamoto 860-8556, Japan.

Country: England

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MEDLINETA: Genes Cells

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