Mass mapping of large globin complexes by scanning transmission electron microscopy.

Mass mapping of large globin complexes by scanning transmission electron microscopy. Research Abstract Details 

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Mass mapping of large globin complexes by scanning transmission electron microscopy. Abstract Text:

Joseph S Wall,Martha N Simon,Beth Y Lin,Serge N Vinogradov,

Scanning transmission electron microscopy (STEM) of unstained, freeze-dried biological macromolecules in the dark-field mode provides an image based on the number of electrons elastically scattered by the constituent atoms of the macromolecule. The image of each isolated particle provides information about the projected structure of the latter, and its integrated intensity is directly related to the mass of the selected particle. Particle images can be sorted by shape, providing independent histograms of mass to study assembly/disassembly intermediates. STEM is optimized for low-dose imaging and is suitable for accurate measurement of particle masses over the range from about 30kDa to 1,000MDa. This article describes the details of the method developed at the Brookhaven National Laboratory STEM facility and illustrates its application to the mass mapping of large globin complexes.

Mass mapping of large globin complexes by scanning transmission electron microscopy. Publishing Authors By Initials

JS Wall,MN Simon,BY Lin,SN Vinogradov,

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Mass mapping of large globin complexes by scanning transmission electron microscopy. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Methods in enzymology

VOLUME: 436C

Page Numbers: 487-501

Journal Abbreviation: Meth. Enzymol.

ISSN: 0076-6879

DAY: 1

MONTH: 02

YEAR: 2008

Mass mapping of large globin complexes by scanning transmission electron microscopy. Information

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LANGUAGE: eng

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AFFILIATION: Biology Department, Brookhaven National Laboratory, Upton, New York.

Country: United States

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MEDLINETA: Methods Enzymol

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